Introduction
This product supplies a simple and rapid extraction of total RNA from plant and fungal samples. The kit is based on super paramagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction process takes only 60 minutes. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing and so on. MagPure RNA Kits buffers can be used for both manual extraction process and automatic nucleic acid extraction machines. This Kits is suitable for extracting RNA from ≤5x106 cultured cells, 20mg tissue and <50mg plant samples.
Details
Specifications
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Features
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Specifications
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Main Functions
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Isolation total RNA from 50mg plant using magnetic particles
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Applications
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RT-PCR, cDNA synthesis, second generation sequencing
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Purification method
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Polydisperse magnetic beads
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Purification technology
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Magnetic beads technology
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Process method
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Manual or automatic
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Sample type
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Plant and fungus samples
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Sample amount
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≤50mg
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Yield
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2-100μg
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Time per run
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≤60 minutes
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Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally RNA was eluted by Elution Buffer.
Technical Validation
MagPure Plant RNA Kit was evaluated as a magnetic bead-based RNA extraction workflow for plant samples, with emphasis on DNA removal, lysis-buffer compatibility and downstream automation readiness. The validation used 50 mg Epipremnum aureum leaf input, magnetic bead purification, 70 µL elution volume and Nanodrop plus agarose gel electrophoresis analysis.
In the DNase I treatment workflow, 50 mg of Epipremnum aureum leaf tissue was processed using both RLC and PAL lysis routes. RLC-based extraction produced RNA yields of 3.22–3.53 µg, with A260/280 values of 2.09–2.13 and A260/230 values of 1.86–1.94. PAL-based extraction produced RNA yields of 3.47–3.55 µg, with A260/280 values of 2.04–2.10 and A260/230 values of 1.86–1.98, indicating high-quality RNA suitable for downstream applications.
The comparison between RLC and PAL lysis showed no obvious difference in RNA yield or purity in the tested plant sample, supporting the use of alternative lysis routes depending on plant matrix behavior. This is consistent with the kit design, where Buffer RL is used as the routine lysis buffer and Buffer PRC1 / PAL-type lysis may be selected when plant secondary metabolites affect sample processing.
Agarose gel electrophoresis showed effective removal of genomic DNA after DNase I treatment, with no obvious DNA residue observed in the tested samples. Together with the magnetic particle workflow and KingFisher-compatible plate setup, these results support R6641 for routine plant RNA extraction where automation, reproducible magnetic handling and integrated DNA removal are required.
Kit Contents
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Contents
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R664101
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R664102
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R664103
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Purification Times
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48 Preps
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96 Preps
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480 Preps
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MagPure RNA Particles
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1.7 ml
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3.5 ml
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18 ml
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DNase I
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600 μl
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2 x 600 μl
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10 x 600 μl
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DNase Buffer
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30 ml
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40 ml
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200 ml
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Buffer PRC1
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40 ml
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70 ml
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350 ml
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Buffer RL
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40 ml
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70 ml
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350 ml
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Buffer MCB*
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18 ml
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30 ml
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150 ml
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Buffer MW1*
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22 ml
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44 ml
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220 ml
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Buffer MW2*
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20 ml
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50 ml
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2 x 100 ml
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RNase Free Water
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10 ml
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15 ml
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120 ml
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Storage and Stability
MagPure RNA Particles should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under theseconditions.
Purchase Guide
MagPure Plant RNA Kit (R6641) is suitable for routine plant samples and laboratories requiring automated or high-throughput RNA extraction workflows.
For manual workflows, column-based systems such as HiPure Plant RNA Mini Kit (R4151) may be used.
For difficult plant samples, CTAB-based workflows such as HiPure HP Plant RNA Mini Kit (R4165) can be considered.
For a complete selection guide:
👉 Plant DNA and RNA Extraction Selection Guide
