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MagPure FFPE DNA/RNA Kit R6327

CAT NO PRODUCT NAME SIZE PRICE
R632701 MagPure FFPE DNA/RNA Kit 48 preps $398.00
R632702 MagPure FFPE DNA/RNA Kit 96 preps $696.00
R632703
MagPure FFPE DNA/RNA Kit
5 x 96 preps
$2,959.00

Introduction

The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and pyrosequencing.

Details


Specifications

Features Specifications
Main Functions Co-isolation DNA and RNA from FFPE tissue
Applications RT-PCR, cDNA synthesis, PCR and second-generation sequencing, etc.
Purification method

Polydisperse silicon based magnetic beads (DNA)

Monodisperse carbonyl magnetic beads (RNA)

Purification technology Magnetic beads technology
Process method Manual or automatic
Adaptive instrument
Nucleic acid extractor and pipetting workstation
Sample type FFPE slice, FFPE puncture sample, embedded tissue
Sample amount No more than six 10 µm sections of 150 mm2 surface area or three 20µm sections of 150 mm2 surface area
Yield DNA: 1 - 10 μg,  RNA: 1 - 25 μg


Principle

The sample is lysed and digested under the action of lysate and protease. DNA/RNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, the supernatant contain RNA was collected and bind to MagBind Particles. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Elution Buffer.


Advantages

  • High quality - high purity total RNA / DNA can be directly used in various downstream applications
  • Safe - no phenol chloroform extraction required
  • Simultaneous extraction - simultaneously isolate DNA and RNA from one sample
  • Post digestion sorting - higher DNA and RNA yields
  • High RNA yield - monodisperse carbonyl adsorption


Kit Contents

Contents R632701 R632702 R632703
Purification Times 48 Preps 96 Preps
5 x 96 Preps
MagBind Particles 1.1 ml 2.5 ml
11 ml
MagPure Particles N 1.1 ml 2.5 ml
11 ml
Proteinase K
24 mg
48 mg
220 mg
Protease Dissolve Buffer
3 ml
10 ml
15 ml
Buffer DPS
50 ml
100 ml
2 x 250 ml
Buffer ATL 20 ml 30 ml
120 ml
Buffer BST1 20 ml 40 ml
200 ml
Buffer BXW1 44 ml 110 ml
3 x 110 ml
RNase Free Water
15 ml
30 ml
120 ml


Storage and Stability

Proteinase K and MagBind Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.


Experiment Data


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