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Your present location:Home/Products/DNA&RNA Purification/RNA/Tissue/Cell RNA/Magnetic Kits/MagPure Total RNA Kit
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MagPure Total RNA Kit

R6622
CAT NO PRODUCT NAME SIZE PRICE
R662201 MagPure Total RNA Kit 48 preps $348.00
R662202 MagPure Total RNA Kit 96 preps $597.00
R662203 MagPure Total RNA Kit
5 x 96 preps
$2686.00

Introduction

This product supplies a simple and rapid extraction of total RNA from tissue and culture cells samples. The kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction processis only 60 minutes. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing and so on. MagPure LQ Kit buffers can be used for both manual extraction process and automatic nucleic acid extraction machines.

Details


Specifications

Features Specifications
Main Functions Isolation total RNA from tissue and cells and plant
Applications RT-PCR, cDNA synthesis, second generation sequencing
Purification method Polydisperse magnetic beads
Purification technology Magnetic beads technology
Process method Manual or automatic
Adaptive instrument
Nucleic acid extractor, pipetting workstation
Sample type Animal tissue, human tissue, cultured cells, lymphocytes, ordinary plant tissue
Sample amount

Cells: 1 x 107

Animal tissue: 20mg

Plant tissue: 100mg

Yeast cell: 5 x 106

Yield 5-100μg


Principle

The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water.


Advantages

  • High purity - OD 260 / 280 :1.9-2.0, OD 260 / 230 :1.5-2.0
  • Economy - less than 50% of the price of Qiagen and other imported products
  • Automatic - extraction without manual participation, saving time and effort
  • Universal - suitable for various animal tissues, cultured cells and conventional plant fungal tissues


Kit Contents

Contents R662201 R662202 R662203
Purification Times 48 Preps 96 Preps
5 x 96 Preps
MagPure RNA Particles 1.7 ml 4.0 ml
18 ml
Proteinase K
24 mg
50 mg
240 mg
Protease Dissolve Buffer
1.8 ml
5 ml
15 ml
DNase I 600 μl 2 x 600 μl
10 x 600 μl
DNase Buffer
30 ml
40 ml
200 ml
RTL Lysis Buffer
40 ml
80 ml
400 ml
Buffer MCB* 18 ml 30 ml
150 ml
Buffer MW1*
44 ml
66 ml
2 x 220 ml
Buffer RW2*
20 ml
50 ml
2 x 100 ml
RNase Free Water
10 ml
30 ml
120 ml


Storage and Stability

MagPure RNA Particles and Proteinase K should be stored at 2–8°C upon arrival. DNase I shouldbe stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles and Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for atleast 18 months under these conditions.

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