| CAT NO | PRODUCT NAME | SIZE | PRICE |
| C13121 | HiPure DNA Midi Column III | 100/Bag | $572.00 |
This column is optimized for plasmid preparation at the midi scale. Compared with general genomic DNA columns, it provides higher binding capacity to accommodate plasmid-rich lysates.
It is typically used in molecular cloning workflows where yield consistency is more critical than sample diversity.
Specifications
| Features | Specifications |
| Recommended application | Plasmid Medium Yield preparation |
| Preservation conditions | Room temperature |
| Stability | Up to 4 years |
| Filter membrane | High quality glass fiber filter GF/B, 8 layers |
| Membrane aperture | 1.0 μm |
| Maximum binding yield of plasmid | 250 μg |
| Maximum yield of alcohol mediated Binding | 1 mg |
|
Plasmid Yields |
Up to 0.25mg |
|
Single liquid carrying capacity of column |
4 ml |
|
Minimum elution volume |
500 μl |
|
Withstand centrifugal force |
5,000 x g |
|
Centrifuge |
Lowspeed centrifuge for 15ml centrifuge tubes, >3000 x g, swing-out Rotor, or Fixed Angle Rotor |
Adsorption Mechanism
Based on the negatively charged DNA skeleton, it has a high affinity for positively charged glass fibers. In high salt and ethanol solutions, DNA/RNA binds to glass fiber and interacts with hydrophilic matrix on silica through hydrogen bond. DNA/RNA is tightly bound. All pollutants can be removed by washing solution. At high salt concentration, nucleic acids selectively bind to silica gel membrane, while other pollutants, mainly proteins, are removed by membrane washing.
Ordering information
| CAT.No. |
Product Name |
Package |
| C13121 |
HiPure DNA Midi Column III (8 x GF/B) with 15ml Collection Tubes |
100/Bag |
HiPure DNA Midi Column III (C13121) is designed for moderate-scale DNA purification with increased binding capacity compared to Mini formats.
For routine laboratory workflows, HiPure DNA Mini Column (C13100) provides a more balanced and efficient solution.
For large-scale DNA preparation requiring higher yield, HiPure DNA Maxi Column (C13122) can be considered.
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