| CAT NO | PRODUCT NAME | SIZE | PRICE |
| 1292750 | MagPure Circulating DNA Rich Maxi Kit | 50 preps | $1094.00 |
| 12927200 |
MagPure Circulating DNA Rich Maxi Kit |
200 preps |
$3978.00 |
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This kit is designed to rich and extract 100bp-500bp circulating DNA from 5 ml cell-free body fluids (such asplasma, serum), and remove fragments above 500bp. Machine reaction only takes 90 minutes. Magnetic-particle technology provides high-quality DNA that is suitable for direct use in downstream applications such as PCR and next generation sequencing.
Specifications
| Features | Specifications |
| Main Functions | Isolation circulatingDNA from 5ml plasma, serum, body fluids |
| Applications | qPCR, NGS, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Serum, plasma |
| Sample amount | 5ml |
| Elution volume | ≥40μl |
| Time per run | ≤50 minutes |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by elution buffer.
| Contents | 1292750 | 12927200 |
| Purification Times | 50 | 200 |
| MagPure Particles G |
20 ml |
80 ml |
| Magbind Particles (selection particles) |
14 ml |
58 ml |
| Selection Solution |
100 ml |
400 ml |
| Proteinase K |
300 mg |
1.2 g |
| Protease Dissolve Buffer |
25 ml |
100 ml |
| Buffer SDS(20%) |
15 ml |
60 ml |
|
Buffer MLK |
300 ml |
3 x 450 ml |
| Buffer BST1 |
225 ml |
2x 450 ml |
|
Buffer MKW1 |
225 ml |
2x 450 ml |
|
Buffer MW2 |
50 ml |
2x 100 ml |
|
Buffer AE |
10 ml |
30 ml |
Storage and Stability
After receive the kit, store the MagPure Particles G, MagBind Particles and Proteinase K at 4°C. Other kit components can be stored at room temperature (15−25°C). Dissolved Proteinase K should be store at -20°C. The reagents are stable for up to one year from the manufacturing date.
