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HiPure FFPE DNA Kit D3126

CAT NO PRODUCT NAME SIZE PRICE
D312602 HiPure FFPE DNA Kit 50 preps $124.00
D312603 HiPure FFPE DNA Kit 250 preps $497.00

Introduction

HiPure FFPE Nucleic acid Kit supplies a simple and rapid DNA extraction for formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 20 minutes (not including digestion time). DNA can be directly used for downstream applications such as PCR, southern blot and viral DNA detection, etc.

Details


Specifications

Features Specifications
Main Functions Isolation total DNA from FFPE tissue samples
Applications PCR, southern blot and viral DNA detection, etc.
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples
Sample amount <20mg
Elution volume <20µl
Time per run 20 minutes
Liquid carrying volume per column 4ml
Binding yield of column 100μg


Principle

Hipure FFPE Nuclear acid kit adopts silica gel column purification. The sample is deparaffinated by xylene and digested by lysate and protease. After de crosslinked at 90 ℃, DNA/RNA is released into the lysate. Adding ethanol to adjust the binding conditions, the sample is transferred to the column where DNA/RNA is adsorbed on the membrane and protein is removed without adsorption. Protein and other impurities are washed by buffer GW1, and the salt is removed by buffer GW2. Finally, the DNA / RNA is eluted by low salt buffer.

Advantages

  • Safety - deparaffinating without contacting with xylene or other toxic solution
  • Fast - without overnight incubation and digestion, several samples can be extracted within 2hours
  • High efficiency -remove the formaldehyde modification of DNA, greatly enhancing the sensitivity of PCR


Kit Contents

Contents D312602 D312603
Purification Times 50 Preps 250 Preps
HiPure DNA Mini Columns I 50 250
2ml Collection Tubes 50 250
Buffer DPS
30 ml
150 ml
Buffer ATL
15 ml
60 ml
Buffer AL 15 ml 60 ml
Buffer GW1* 22 ml 88 ml
Buffer GW2* 12 ml 50 ml
Proteinase K 24 mg 120 mg
Protease Dissolve Buffer 1.8 ml 10 ml
Buffer AE 10 ml 30 ml

Storage and Stability

Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.


Experiment Data


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