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HiPure DNA Micro Kit D3125

CAT NO PRODUCT NAME SIZE PRICE
D312502 HiPure DNA Micro Kit 50 preps $149.00
D312503 HiPure DNA Micro Kit 250 preps $696.00

Introduction

This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from small volume of blood, tissue and dry blood spots.

Details


Specifications

Features Specifications
Main Functions Isolation total DNA from 1-10μl blood, <2mg tissue and other samples
Applications PCR, southern bolt and virus detection, etc.
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Animal tissues, blood spots, swabs and various forensic samples
Sample amount Blood:1-10μl, Tissue:<2mg
Elution volume
Time per run
Liquid carrying volume per column
Binding yield of column


Principle

This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mmTris, pH9.0, 0.5mm EDTA).


Advantages

  • Fast - several samples can be extracted in 20 minutes (after digestion)
  • High purity - purified DNA can be directly used in various downstream applications
  • High recovery - DNA can be recovered at the level of PG
  • Good repeatability - silica technology can obtain ideal results every time


Kit Contents

Contents D312502 D312503
Purification Times 50 Preps 250 Preps
Buffer ATL 15 ml 60 ml
Buffer AL 15 ml 60 ml
Buffer GW1 22 ml 66 ml
Buffer GW2 20 ml 2 x 50 ml
Carrier RNA 310 μg 2 x 310 µg
Proteinase K 24 mg 120 mg
Protease Dissolve Buffer
1.8 ml 10 ml
Buffer AE
15 ml
60 ml
HiPure DNA Mini Columns I
50 2 x 125
2 ml Collection Tubes
50 5 x 100


Storage and Stability

RNase A and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.


Experiment Data


Purchase Guide

Name CAT NO

Tissue amount

Cell number

Column type

Elution volume

Yield (liver)

Yield (muscle)

HiPure Tissue DNA Kit D3121 1 - 30mg 5 x 106 1.5ml column 30 - 100μl 35μg/10mg g/10mg
HiPureDNA Micro Kit D3125 <2mg 1 x 105 1.5ml column 15 - 50μl 3.6μg/1mg 0.89μg/1mg

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