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HiPure Viral DNA/RNA Kit

IVD4173 HiPure Viral RNA/DNA Kit 100 preps $249.00


This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, urine, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.



Features Specifications
Main Functions Extract viral RNA/DNA from non-cell/low cell content biological samples
Applications RT-PCR,PCRNGS
Products Viral total nucleic acid
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Cell-free body fluid such as plasma, serum, soaking solution and tissue homogenate supernatant
Sample amount 200μl
Yield 2-10μg
Elution volume 30μl
Time per run 30 minutes
Liquid carrying volume per column 800μl
Binding yield of column 100μg


This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA / RNA is released into the lysate. Transfer to an adsorption plate and filter column. DNA/RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, DNA / RNA was finally eluted with low-salt buffer (10 Mm Tris, pH 8.0).


  • Fast - several samples can be extracted in 20 minutes by column method
  • High quality - high purity total RNA can be directly used in various sensitive downstream applications
  • Safe - no phenol chloroform extraction required
  • Sensitive - DNA/RNA can be recovered at the level of PG
  • High yield - carrier RNA contained in the product maximize the recovery of trace nucleic acid

Kit Contents

Contents IVD4173
Purification Times 100 Preps
HiPure Viral Mini Column 100
2ml Collection Tubes
PK/Carrier RNA 50 mg
Protease Dissolve Buffer 5 ml
Buffer AL
30 ml
Buffer MW1*
44 ml
Buffer MW2*
50 ml
RNase Free Water
15 ml

Storage and Stability

Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.

Experiment Data

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