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Your present location:Home/Products/DNA&RNA Purification/RNA/Viral/Pathogen DNA/RNA/HiPure Pathogen DNA/RNA Kit (NGS)
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HiPure Pathogen DNA/RNA Kit (NGS)

IVD4179
CAT NO PRODUCT NAME SIZE PRICE
IVD4179 HiPure Pathogen DNA/RNA Kit (NGS) 50 preps $429.00

Introduction

This kit is used for extracting total pathongen nucleic acid from low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate, culture, etc. The purified DNA/RNA is ready for downstream clinical in vitro detection such as Real Time PCR, biochip analysis, NGS and other related detection.

Details


Specifications

Features Specifications
Main Functions Extract pathogen DNA/RNA from whole blood, body fluid, serum, plasma, sputum, immersion solution, tissue homogenate supernatant, etc.
Applications RT-PCR,PCRNGS
Products Pathogen DNA and RNA
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Whole blood, plasma, serum, immersion solution, tissue homogenate supernatant
Sample amount 200μl
Elution volume 30μl
Time per run 30 mins
Liquid carrying volume per column 800μl
Binding yield of column 100μg


Principle

This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA/RNA is released into the lysate. Transfer to an adsorption plate and filter column. DNA/RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, DNA / RNA is finally eluted with low-salt buffer (10 MmTris, pH 8.0).


Advantages

  • Fast - column purification, several samples can be finished in 30 mins
  • High quality - high purity total RNA/DNA can be directly used in various sensitive downstream applications
  • Safe - no phenol chloroform extraction required
  • Sensitive - DNA/RNA can be recovered at the level of PG


Kit Contents

Contents IVD4179
Purification Times 50 Preps
HiPure Viral Mini Column 50
2ml Collection Tubes
100
2ml Bead Tubes
50
Protease K 50 mg
Protease Dissolve Buffer 5 ml
DNase I (Powder)
10 mg
DNase Buffer
6 ml
Buffer CLB
100 ml
Buffer SDS
5 ml
Reagent DX
1.5 ml
Buffer ACL
30 ml
Buffer VHB*
22 ml
Buffer RW2*
20 ml
Buffer AVE
15 ml


Storage and Stability

Proteinase K and DNase I (Powder) should be stored at 2–8°C upon arrival. However, short-term storage (DNase I up to 1 week, Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affecttheir performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.

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