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HiPure Cell miRNA Kit

R431102 HiPure Cell miRNA Kit 50 preps $174.00
R431103 HiPure Cell miRNA Kit 250 preps $746.00


The Kit is designed for purification of total RNA, including miRNA and other small RNA molecules (18nt), from cultured cells and various animal and human tissues, including difficult-to-lyse tissues samples.



Features Specifications
Main Functions Isolation total RNA and miRNA from cell and tissue without MagZol reagent
Applications RT-PCR, Northern Blot, poly A+purification, nucleic acid protection and in vitro translation
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Cell, Animal tissue, Plant tissue
Sample amount Cells: ≤ 5 x 10^6, Animal tissue:<10mg
Elution volume ≥30μl
Time per run ≤40 minutes
Liquid carrying volume per column 800µl
Binding yield of column 100µg


Biological samples are first lysed and homogenized in a highly denaturing guanidine isothiocyanate-containing buffer, which immediately inactivates DNases and RNases to ensure isolation of intact DNA and RNA. The lysate is then passed through a Mini spin column. This column, in combination with the high-salt buffer, allows selective and efficient binding of genomic DNA. Flow-through from the column is digested by Proteinase K in the presence of ethanol. This optimized digestion, together with the subsequent addition of further ethanol, allows appropriate binding of total RNA, including miRNA, to the column. Contaminants are efficiently washed away and high-quality RNA is eluted.


  • High quality - one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
  • Fast - several samples can be extracted in 40 minutes
  • High applicability - samples including animals, plants, bacteria, cells, etc.
  • High concentration - efficiently remove macromolecular RNA, enrich small RNA and improve sensitivity
  • Safe - no phenol chloroform extraction and no use of Trizol reagent

Kit Contents

Contents R431102 R431103
Purification Times 50 Preps 250 Preps
HiPure RNA Mini Columns 100 2 x 250
2ml Collection Tubes
2 x 250
Proteinase K
48 mg
240 mg
Protease Dissolve Buffer
5 ml
15 ml
Buffer RLC
40 ml
200 ml
Buffer RWC
20 ml
80 ml
Buffer RW2*
20 ml
2 x 50 ml
RNase Free Water
10 ml
60 ml

Storage and Stability

Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.


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