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HiPure Liquid RNA (miRNA) Kit

R416302 HiPure Liquid RNA (miRNA) Kit 50 preps $124.00
R416303 HiPure Liquid RNA (miRNA) Kit 250 preps $547.00


The HiPure Liquid RNA & miRNA Kit integrates phenol/guanidine-based sample lysis and silica membrane purification of total RNA. MagZol LS Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate designed to facilitate lysis of liquid sample and inhibit RNase. The high lysis efficiency of the reagent and the subsequent removal of contaminants by organic phase extraction enable the use of up to 0.25ml liquid sample per mini spin column.



Features Specifications
Main Functions Isolation total RNA from 0.25ml body fluids using column and MagZol LS reagent
Applications qPCR / RT-PCR, liquid or solid-phasechip analysis, hybridization and SNP detection
Purification method Mini spin column
Purification technology Silica technology, acidphenol / guanidine extraction technology (MagZol pretreatment technology)
Process method Manual (centrifugation or vacuum)
Sample type Blood, serum, plasma
Sample amount 250μl
Elution volume 20μl
Liquid carrying volume per column 800µl
Binding yield of column 100μg


0.25ml liquid samples are homogenized in 0.75ml MagZol LS Reagent. After addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. The upper, aqueous phase is extracted, and ethanol is added to provide appropriate binding conditions. The sample is then applied to the spin column, where the total RNA (up to 100 µg) binds to the membrane and phenol and other contaminants are efficiently washed away. High-quality RNA is then eluted in 30–100µl of RNase-free water.


  • High quality - high purity total RNA can be directly used in various sensitive downstream applications
  • Fast – isolation of several samples can be completed in 40 minutes by using column purification method
  • Safety - no phenol chloroform extraction required
  • Sensitive - direct lysis of blood, plasma and other samples without separation of leukocytes

Kit Contents

Contents R416302 R416303
Purification Times 50 Preps 250 Preps
HiPure RNA Mini Columns 50 250
2ml Collection Tubes
2 x 125
MagZol LS Reagent
60 ml
270 ml
Buffer RWC 20 ml 60 ml
Buffer RW2*
20 ml
2 x 50 ml
RNase Free Water
10 ml
30 ml

Storage and Stability

MagZol LS Reagent should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

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