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HiPure Paxigene Blood RNA Kit

R416802 HiPure Paxigene Blood RNA Kit 10 preps $99.00
R416803 HiPure Paxigene Blood RNA Kit 50 preps $373.00


The Kit provides fast purification of high-quality RNA from paxigene Blood RNA Tube using silica-membrane spin columns. RNA purified using the HiPure System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.



Features Specifications
Main Functions Isolation total RNA from Paxgene RNA Tubes
Applications RT-PCR, qPCR, Northern hybridization, NGS, nucleic acid protection, in vitro translation
Purification method Mini spin column
Purification technology Silica technology, DNA filtration technology
Process method Manual (centrifugation or vacuum)
Sample type Blood in the preservation tube
Sample amount 2.5ml
Elution volume 30μl
Liquid carrying volume per column 800µl
Binding yield of column 100μg


The Kit is for the purification of total RNA from 2.5 ml human whole blood collected in a PAXgene Blood RNA Tube. Purification begins with a centrifugation step to pellet nucleic acids in the PAXgene Blood RNA Tube. The pellet is washed and resuspended, and incubated in optimized buffers together with proteinase K. DNA wash The lysate is passed through a DNA Mini column. Ethanolis added to adjust binding conditions, and the lysate is applied to a column.RNA is selectively bound to the silica membrane as contaminants pass through. Remaining contaminants are removed in several efficient wash steps. Between the first and second wash steps, the membrane is treated with DNase I to remove trace amounts of bound DNA. After the wash steps, RNA is eluted in elution buffer and heat-denatured.


  • High quality - high purity total RNA can be directly used in various sensitive downstream applications
  • Fast - isolation of several samples can be completed in 40 minutes by using column purification method
  • Safety - no phenol chloroform extraction required
  • Sensitive - direct lysis of blood, plasma and other samples without separation of leukocytes

Kit Contents

Contents R416802 R416803
Purification Times 10 Preps 50 Preps
HiPure RNA Mini Columns I 10 50
gDNA Filter Mini Columns
2ml Collection Tubes
RNase Free Water 60 ml 250 ml
Buffer MBR1
10 ml
30 ml
Buffer MBR2
5 ml 15 ml
Buffer RW1
15 ml
60 ml
Buffer RW2*
6 ml
20 ml
Proteinase K
12 mg
50 mg
Protease Dissolve Buffer
1.8 ml
5 ml
DNase I
120 µl
600 µl
DNase Buffer
6 ml
30 ml

Storage and Stability

Proteinase K should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

Experiment Data

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