Introduction
The HiPure Total RNA Plus Kit (R4111) is designed for purification of high-quality total RNA from animal tissues and cultured cells using silica membrane spin column technology. The system integrates an additional genomic DNA removal step that eliminates genomic DNA contamination prior to RNA purification, enabling reliable RNA preparation for downstream gene expression analysis.
The extraction workflow combines efficient sample lysis with silica membrane RNA adsorption to selectively purify RNA molecules while removing proteins, genomic DNA and other contaminants. The resulting RNA is suitable for RT-PCR, transcriptomic analysis and other RNA-based molecular biology workflows.
Within the Magen RNA extraction systems, the HiPure Total RNA Plus Kit serves as a core column-based solution for high-purity RNA preparation when removal of genomic DNA contamination is critical for downstream applications.
Details
Specifications
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Features
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Specifications
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Main Functions
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Isolation total RNA (not include miRNA) from 20mg tissue, 150mg plant, 5 x 106 cell using two columns (gDNA removed column)
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Applications
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RT-PCR, qRT-PCR, Northern hybridization, second generation sequencing
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Purification method
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Mini spin column
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Purification technology
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Silica technology, DNA filtration technology
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Process method
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Manual (centrifugation or vacuum)
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Sample type
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Animal soft tissue, cultured cells, lymphocytes, simple plant tissue
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Sample amount
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Cells:≤1 x 107
Animal tissue sample: 1-20 mg
Plant tissue: 50-150 mg
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Yield
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2-100μg
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Elution volume
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≥50μl
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Time per run
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≤25 minutes（1-24 samples）
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Liquid carrying volume per column
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800µl
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Binding yield of column
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100µg
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Engineering Characteristics
Integrated genomic DNA removal
The workflow includes a dedicated gDNA removal column that eliminates genomic DNA contamination before RNA purification, reducing the need for additional DNase treatment.
Efficient RNA binding chemistry
Silica membrane adsorption enables selective binding of RNA molecules while cellular proteins and other contaminants are removed during washing steps.
Optimized lysis and inhibitor removal
The extraction buffers efficiently disrupt cellular structures and remove contaminants that may interfere with downstream reverse transcription reactions.
Spin-column workflow
The purification procedure is compatible with routine laboratory centrifuges and supports rapid RNA purification workflows in research laboratories.
Technical Validation
Validation experiments using animal tissue samples demonstrated stable RNA purification with purity ratios A260/280 around 2.0–2.1 and A260/230 values around 2.0–2.2, indicating high-quality RNA suitable for downstream gene expression analysis.
RNA yields obtained from tissue samples were consistent across replicate extractions and comparable to commonly used commercial RNA purification systems in parallel experiments.
Electrophoresis analysis confirmed intact RNA bands without detectable degradation, supporting compatibility with RT-PCR and transcriptomic workflows.
Typical Applications
Total RNA purified using the HiPure Total RNA Plus Kit is suitable for a wide range of RNA analysis workflows, including:
• RT-PCR analysis
• Gene expression analysis
• Transcriptomics studies
• RNA sequencing sample preparation
• Molecular diagnostics research
• Functional genomics studies
Related Products
• HiPure Universal RNA Kit (R4130) – purification of total RNA including small RNA fractions using MagZol reagent chemistry
• MagPure Universal RNA Kit (IVD3020) – magnetic bead-based automated purification of RNA from tissue and cultured cells
• MagPure Blood RNA Kit (R6611) – magnetic bead purification of RNA from whole blood samples
• HiPure Universal miRNA Kit (R4310) – enrichment and purification of small RNA molecules from tissue samples
Kit Contents
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Contents
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R411102
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D411103
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Purification Times
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50 Preps
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250 Preps
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HiPure DNA Mini Columns
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50
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250
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HiPure RNA Mini Columns
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50
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250
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2ml Collection Tubes
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100
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2 x 250
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Buffer RLC
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50 ml
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200 ml
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Buffer RW1
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50 ml
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200 ml
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Buffer RW2*
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12 ml
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2 x 50 ml
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RNase Free Water
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10 ml
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30 ml
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Storage and Stability
HiPureKit can be stored dry at room temperature (15-25°C) and are stable for at least18 months under these conditions. During shipment, crystals or precipitationmay form in the Buffer RLC. Dissolve by warming buffer to 37°C.
