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HiPure Universal RNA Kit

R413002 HiPure Universal RNA Kit 50 preps $124.00
R413003 HiPure Universal RNA Kit 250 preps $547.00


The Kit integrates phenol/guanidine-based lysis and silica membrane purification of total RNA. MagZol Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate lysis of samples and inhibit RNases. The high lysis efficiency of the reagent and the subsequent removal of contaminants by organic phase extraction enable extracting up to 200μg total purified RNA from less than 100mg animal, plant, fungal samples,bacteria, cells and other samples.



Features Specifications
Main Functions Isolation total RNA (include miRNA) from 100mg lipid tissue, tissue, cell, plant, body fluids using columns and MagZol reagent
Applications RT-PCR, qRT-PCR, Northern hybridization, second generation sequencing
Purification method Mini spin column
Purification technology Silica technology, acid phenol / guanidine extraction technology (MagZol pretreatment technology)
Process method Manual (centrifugation or vacuum)
Sample type Animal tissue, muscle fiber containing tissue,adipose tissue, cultured cells, lymphocytes, simple plants and other biological samples
Sample amount

Animal tissue sample: 1-60mg (spleen10mg)

Cultured cells: 5 x 106

Plant leaves: 50-100mg

Yield 2-200μg
Elution volume 50μl
Time per run 30 minutes(1-24 samples
Liquid carrying volume per column 800µl
Binding yield of column 100µg


Tissue samples (10-100mg) are homogenized in MagZol Reagent. After addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. The upper, aqueous phase is extracted, and ethanol is added to provide appropriate binding conditions. The sample is then applied to the spin column, where the total RNA (up to 100µg) binds to the membrane and phenol and other contaminants are efficiently washed away. High-quality RNA is then eluted in 30-100µl of RNase-free water.


  • Efficient DNA removal - one step RNA extraction can effectively remove genomic DNA
  • High quality - one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
  • Fast - several samples can be extracted in 30 minutes
  • High applicability - samples including animals, plants, bacteria, cells, etc.
  • High output - enable to process large samples and the yield can be up to 200μg

Kit Contents

Contents R413002 D413003
Purification Times 50 Preps 250 Preps
HiPure RNA Mini Columns 50 250
2ml Collection Tubes 50 250
MagZol Reagent
60 ml
270 ml
Buffer RW1 50 ml 200 ml
Buffer RW2*
20 ml
50 ml
RNase Free Water
10 ml
30 ml

Storage and Stability

MagZol Reagent should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Experiment Data


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