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Your present location:Home/Products/DNA&RNA Purification/RNA/Tissue/Cell RNA/MagPure Universal RNA Kit
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MagPure Universal RNA Kit

IVD3020
CAT NO PRODUCT NAME SIZE PRICE
IVD3020 MagPure Universal RNA Kit 200 preps $895.00
IVD3020-F-96 MagPure Universal RNA Precast Kit (96 channel machine) 96 preps $550.00
IVD3020-TL-06 MagPure Univseral RNA Precast Kit (32/48 Channel Machine) 96 preps $550.00

Introduction

This product is suitable for rapid RNA extraction from tissue , cells, and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR and so on.

Details


Specifications

Features Specifications
Main Functions Isolation total RNA from tissue, cell
Applications RT-PCR, cDNA synthesis, second generation sequencing
Purification method Polydisperse magnetic beads
Purification technology Magnetic beads technology
Process method Manual or automatic
Adaptive instrument
Nucleic acid extractor, pipetting workstation
Sample type Tissues, cells, lymphocytes and other clinical sample
Sample amount

Cells grown in suspension:3~5 x 106

Animal tissue: 10~20mg

Plant tissue: 100mg


Principle

The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase I. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water


Advantages

  • High purity - OD260 / 280 :1.9-2.0, OD 260 / 230 :1.5-2.0
  • Economy - less than 50% of the price of Qiagen and other imported products
  • Automatic - extraction without manual participation, saving time and effort
  • Universal - suitable for various clinical samples


Kit Contents

Contents IVD3020
Purification Times 200 Preps
MagPure RNA Particles
7 ml
DNase I
4 x 600 µl
DNase Buffer
80 ml
RTL Lysis Buffer
150 ml
Buffer MCB*
75 ml
Buffer MW1* 110 ml
Buffer MW2*
50 ml
RNase Free Water
60 ml
Cat.No Reagent
IVD3020-F-96
DNase Buffer
60 ml
DNase I

2 x 600 μl
RTL Lysis Buffer
80 ml
Buffer MCB
18 ml
96-Tip
1
Sample plate (DW Plate) 500μl Buffer MCB
1
Wash 1 Plate (DW Plate)

700μl Buffer MW1

30μl MagPure RNA Partilces

1
DNase Plate
Empty

Wash 2 Plate (DW Plate) 700μl Buffer MW1
1
Wash 3 Plate (DW Plate) 900μl Buffer MW2
1
Elution plate (DW Plate) 80μl RNase Free Water
1
Cat.No Reagent
IVD3020-TL-06
Purification times
96 Preps
DNase I
2 x 600 μl
DNase Buffer
60 ml
RTL Lysis Buffer
60 ml
Buffer MCB

40 ml
96-Tip
12 PCS

2.0ml V-bottom plate

Row 1/7:500μl Buffer MCB

Row 2/8:500μl Buffer MW1

Row 3/9:empty

Row 4/10:30μl Magpure RNA Particles

500μl Buffer MW2

Row 5/11:900μl Buffer MW2

Row 6/12:80μl RNase Free Water

6


Storage and Stability

MagPure RNA Particles should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles up to 8 weeks) at roomtemperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

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