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HiPure Bacterial DNA Kit

D314602 HiPure Bacterial DNA Kit 50 preps $149.00
D314603 HiPure Bacterial DNA Kit 250 preps $646.00


This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA can be purified from bacterial culture, body fluids, food and fermentation.



Features Specifications
Main Functions Isolation bacterial DNA from cultures, food and other samples
Applications PCR, southern blot and enzyme digestion, etc.
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Culture medium, swab, parasitic blood, tissue,sputum, etc.
Sample amount

Bacterial culture medium: 0.5-2 ml

Tissue samples: 50-100 mg

Whole blood / cell suspension: 0.5-1 ml

Viscous secretion: 0.1-1 g

Elution volume ≥30μl
Time per run 40 minutes
Liquid carrying volume per column 800μl
Binding yield of column 100μg


This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while proteinis not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mmTris, pH9.0, 0.5mm EDTA). This product carries 0.1-0.2mm acid glass beads, which can improve the wall-breaking effect of lysozyme resistant bacteria, the success rate and DNA yield through physical bead grinding.


  • Fast - several samples can be extracted in 40 minutes (after digestion)
  • High purity - purified DNA can be directly used in various downstream applications
  • Good repeatability - silica technology can obtain ideal results every time
  • High recovery - DNA can be recovered at the level of PG
  • Broad spectrum - it can deal with a variety of bacteria, including Gram-positive bacteria that are difficult to disrupt
  • Sufficient components - this kit has carried lysozyme, protease K, RNase A and glass beads

Kit Contents

Contents D314602 D314603
Purification Times 50 Preps 250 Preps
Hipure DNA Mini Columns I 50 2 x 125
2ml Collection Tubes 50 2 x 125
Glass Beads (0.1~0.2mm)
20 g
100 g
Buffer P1
20 ml
100 ml
Buffer DL 15 ml 80 ml
Buffer GW1
22 ml
88 ml
Buffer GW2
12 ml
50 ml
Lysozyme 60 mg 300 mg
Proteinase K 24 mg 120 mg
Protease Dissolve Buffer 5 ml 15 ml
Buffer AE
15 ml
60 ml

Storage and Stability

Lysozyme and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.


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