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HiPure Microbiome DNA Kit

D314802 HiPure Microbiome DNA Kit 50 preps $348.00
D314803 HiPure Microbiome DNA Kit 250 preps $1,641.00


This product provides an easy-to-use workflow for selective isolation of bacterial DNA from samples that are intrinsically rich in host DNA, such as body fluids or swabs. The method is specific for the identification of intact bacteria so it prevents false results due to nucleic acids from dead bacteria. The Kit allows isolation of enriched bacterial DNA suitable for a variety of applications, including qPCR and whole metagenome or 16S rRNA gene sequencing.



Features Specifications
Main Functions Isolation gDNA from biological sample and remove host DNA
Applications PCR, southern blot and enzyme digestion, etc.
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Culture medium, swab, parasitic blood, tissue, sputum, etc.
Sample amount

Blood sample, homogenate, plasma, brain effusion, swab immersion solution, centrifuged concentrated liquid, etc.:0.5-1ml

Elution volume ≥50μl
Time per run 60 minutes
Liquid carrying volume per column 800μl
Binding yield of column 100μg


This product is based on silica Column purification. This product efficiently depletes human and animal host DNA and yields enriched bacterial DNA. An optimized combination of mechanical and chemical lysis allows efficient disruption of bacterial cells. Target DNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).


  • High concentration - the host DNA is digested by dnase, without contamination of host DNA
  • High recovery - DNA can be recovered at the level of PG
  • Good repeatability - silica technology can obtain ideal results every time
  • Wide applicability - it can be used in blood, tissue, intestinal contents and other samples

Kit Contents

Contents D314802 D314803
Purification Times 50 Preps 250 Preps
Hipure DNA Mini Columns I 50 2 x 125
2ml Collection Tubes 50 2 x 125
2ml beads Tubes
Buffer DRB
15 ml
60 ml
Buffer ES 6 ml 30 ml
Reagent DX
0.5 ml
1 ml
Buffer DL
30 ml
120 ml
Buffer GW1
22 ml
110 ml
Buffer GW2
12 ml
50 ml
DNase I (Powder) 6 mg 30 mg
Proteinase K 24 mg 120 mg
Protease Dissolve Buffer 5 ml 15 ml
Buffer AE
15 ml
60 ml

Storage and Stability

DNase I and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.


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