| CAT NO | PRODUCT NAME | SIZE | PRICE |
| D314802 | HiPure Microbiome DNA Kit | 50 preps | $348.00 |
| D314803 | HiPure Microbiome DNA Kit | 250 preps | $1,641.00 |
This product provides an easy-to-use workflow for selective isolation of bacterial DNA from samples that are intrinsically rich in host DNA, such as body fluids or swabs. The method is specific for the identification of intact bacteria so it prevents false results due to nucleic acids from dead bacteria. The Kit allows isolation of enriched bacterial DNA suitable for a variety of applications, including qPCR and whole metagenome or 16S rRNA gene sequencing.
Specifications
| Features | Specifications |
| Main Functions | Isolation gDNA from biological sample and remove host DNA |
| Applications | PCR, southern blot and enzyme digestion, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Culture medium, swab, parasitic blood, tissue, sputum, etc. |
| Sample amount |
Blood sample, homogenate, plasma, brain effusion, swab immersion solution, centrifuged concentrated liquid, etc.:0.5-1ml |
| Elution volume | ≥50μl |
| Time per run | ≤60 minutes |
| Liquid carrying volume per column | 800μl |
| Binding yield of column | 100μg |
This product is based on silica Column purification. Eukaryocyte is lysed in lysis buffer LBX1, but bacterial/fungal will not lysed. Microbial cells gets after centrifuge, microbial DNA release in lysis buffer and proteinase K. Target DNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. Salts and other impurities are remove by wash buffer 2, nucleic acid is finally eluted with low-salt buffer (Buffer AVE).
Kit Contents
| Contents | D314802 | D314803 |
| Purification Times | 50 Preps |
250 Preps |
| Hipure DNA Mini Columns I | 50 |
250 |
| 2ml Collection Tubes | 50 |
250 |
|
2ml beads Tubes |
50 |
250 |
|
Buffer LBX1 |
60 ml |
270 ml |
| DNase Buffer | 15 ml |
60 ml |
|
Buffer TL |
15 ml |
60 ml |
|
Buffer MLB |
30 ml |
150 ml |
|
Buffer DCW1* |
22 ml |
110 ml |
|
Buffer DCW2* |
20 ml |
2 x 50 ml |
| DNase I (Powder) | 10 mg |
4 x 10 mg |
| Proteinase K | 24 mg |
120 mg |
| Protease Dissolve Buffer | 3 ml |
10 ml |
|
Buffer AVE |
10 ml |
30 ml |
Storage and Stability
DNase I and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Select the right purification kit to get impactful results：
Ⅰ. Purchase guide of Magen kits based on sample
Ⅱ. Cross purchase guide for Magen kits vs Other brands
For any customized product or OEM service, please contact us.
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