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HiPure Insect DNA 96 Kit

D3139
CAT NO PRODUCT NAME SIZE PRICE
D313901 HiPure Insect DNA 96 Kit 1 x 96 Preps
D313902 HiPure Insect DNA 96 Kit 4 x 96 Preps

Introduction

HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.

Details


Specifications

Features Specifications
Main Functions Isolation total DNA from insect tissue
Applications PCR, southern bolt and virus detection, etc
Purification method 96 well DNA plate
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Insect tissue samples
Sample amount
Elution volume
Time per run
Liquid carrying volume per column
Binding yield of column


Principles

This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).


Advantages

  • High purity DNA - can be used in sensitive downstream applications such as multiplex and quantitative pcr
  • Good repeatability - suitable for extracting high-yield DNA from insect tissue samples
  • Safe - without phenol chloroform extraction and alcohol precipitation

Kit Contents

Contents D313901 D313902
Purification Times 1 x 96 4 x 96
HiPure DNA Plate 1 4
2.2 ml Collection Plate
1
4
1.6 ml Collection Plate
1
4
0.5ml Collection Plate
1
4
Seal Film 8 32
Buffer ITL 30 ml 120 ml
Buffer IL
30 ml 125 ml
Buffer GW1 44 ml 2 x 110 ml
Buffer GW2 50 ml 3 x 50 ml
Proteinase K 50 mg 200 mg
Protease Dissolve Buffer 6 ml 15 ml
Buffer AE 20 ml 60 ml

Storage and Stability

Proteinase K, RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in thiscase buffers should be redissolved before use. Make sure that all buffers areat room temperature when used.

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