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IVD3126 HiPure FFPE DNA Kit 100 preps $298.00


HiPure FFPE Nucleic acid Kit supplies a simple and rapid DNA extraction for Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 20 minutes (not including digestion time). DNA can be directly used for downstream applications such as PCR, southern blot and viral DNA detection, etc.



Features Specifications
Main Functions Isolation total DNA from FFPE tissue samples
Applications PCR, Southern Blot and viral DNA detection, etc.
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples
Sample amount <20mg
Elution volume <20µl
Time per run 20 minutes
Liquid carrying volume per column 4ml
Binding yield of column 100μg


Hipure FFPE Nuclear acid kit adopts silica gel column purification. The sample is deparaffinated by xylene and digested by lysate and protease. After decrosslinked at 90 ℃, DNA/RNA is released into the lysate. Adding ethanol to adjust the binding conditions, the sample is transferred to the column where DNA/RNA is adsorbed on the membrane and protein is removed without adsorption. Protein and other impurities are washed by buffer GW1, and the salt is removed by buffer GW2. Finally, the DNA / RNA is eluted by low salt buffer.


  • Safety - deparaffinating without contacting with xylene or other toxic solution
  • Fast - without overnight incubation and digestion, several samples can be extracted within 2hours
  • High efficiency - remove the formaldehyde modification of DNA, greatly enhancing the sensitivity of PCR
  • High yield - most optimal process to ensure the highest recovery
  • High recovery - silica gel column purification method can recover nucleic acid at the level of PG

Kit Contents

Contents IVD3126
Purification Times 100 Preps
HiPure DNA Mini Columns I 100
2ml Collection Tubes 100
Buffer DPS
70 ml
Buffer ATL
30 ml
Buffer AL 30 ml
Buffer GW1* 44 ml
Buffer GW2* 20 ml
Proteinase K 50 mg
Protease Dissolve Buffer 6 ml
Buffer AE 20 ml

Storage and Stability

Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

Experiment Data

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