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Your present location:Home/Products/DNA&RNA Purification/DNA/Circulating/Cell-free/Cf DNA/MagPure Circulating DNA Rich Mini Kit
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MagPure Circulating DNA Rich Mini Kit

12917
CAT NO PRODUCT NAME SIZE PRICE
1291750 MagPure Circulating DNA Rich Mini Kit 50  preps $199.00
12917200 MagPure Circulating DNA Rich Mini Kit
200 preps $696.00




Introduction

MagPure Circulating DNA Rich Kit designed for purification of high quality circulating DNA (cfDNA) from cell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.

Details


Specifications

Features Specifications
Main Functions Rich small fragment cfDNA from 0.6ml serum plasma, remove DNA fragments>500bp
Applications qPCR, NGS, etc.
Purification technology Magnetic beads technology
Process method Manual or automatic
Sample type Serum, plasma
Sample amount 0.6ml
Elution volume
Time per run


Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysedand digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, Large DNA(>500bp) will be adsorbed on the surface of magBindparticles. After removal of large size, small size of DNA(<500bp) will be adsorbed on the surface ofMagPure Particle F and impurities such as proteins will be removed without adsorption. The adsorbedparticles were washed with washing solution to remove proteins and impurities, washed with ethanol toremove salts, and finally DNA

Advantages

  • Economy - less than 50% of the price of Qiagen and other imported products
  • Automatic - without labour


Kit Contents

Contents 1292750 12927200
Purification Times 50 preps 200 preps
MagPure Particles F 1.2 ml
4.5 ml
MagBind Particles 1.2 ml
4.5 ml
Proteinase K 24 mg 90 mg
Protease Dissolve Buffer 1.8 ml
10 ml
Buffer MLB
30 ml
120 ml
Buffer GDP 15 ml
60 ml
Buffer MAW1
40 ml
250 ml
Buffer MW2*
20 ml
50 ml
Elution Buffer
15 ml
60 ml

Storage and Stability

Proteinase K, MagBind Particles and MagPure Particles F should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

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