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HiPure Circulating DNA Midi Kit C (Vaccum Protocol)

D3182C
CAT NO PRODUCT NAME SIZE PRICE
D318201C HiPure Circulating DNA Midi Kit C 10 preps $136.00
D318202C
HiPure Circulating DNA Midi Kit C
50 preps
$547.00

Introduction

Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments, <1000bp (DNA). HiPure Circulating DNA Midi Kit enables efficient purification of these circulating nucleic acids from human plasma, serum, or urine.

This product is for research use only.


Video

Details


Specifications

Features Specifications
Main Functions Isolation circulating DNA from 1-5ml plasma, serum, body fluids using vacuum protocol
Applications qPCR, liquid or solid chip analysis, hybridization and SNP detection, etc.
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (vacuum)
Sample type Serum, plasma and other cell-free fluid samples
Sample amount 1-5ml
Elution volume ≥50μl
Time per run ≤60 minutes
Liquid carrying volume per column 4ml
Binding yield of column 1mg


Principle

This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer.

Advantages

  • High yield - most optimal process, free DNA (>50bp) can be obtained to the maximum extent
  • High concentration - low elution volume, ensuring high nucleic acid concentration
  • High purity - low alcohol binding method, completely removing inhibitor and protein pollution
  • High recovery - DNA can be recovered at thelevel of PG by silica gel column purification


Kit Contents

Contents D318201C
D318202C
Purification Times 10
50
Buffer ACL 50 ml
250 ml
Buffer ACB* 60 ml
300 ml
Buffer DCW1* 4.4 ml
22 ml
Buffer DCW2* 5 ml
10 ml
Proteinase K 110 ml
540 mg
Protease Dissolve Buffer 10 ml
30 ml
Carrier RNA 110 μg
110 μg
Nuclease Free Water
10 ml
20 ml
HiPure CFDNA Mini Columns
10
50
2 ml Collection Tubes
20
100
Extender Tube
10
50
Vac-Connector
10
50


Storage and Stability

Proteinase K, Carrier RNA should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.


Experiment Data





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