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Your present location:Home/Products/DNA&RNA Purification/DNA/RNA Coisolation/DNA/RNA Coisolation/HiPure FFPE DNA/RNA Kit
  • IVD5116.png

HiPure FFPE DNA/RNA Kit

IVD5116
CAT NO PRODUCT NAME SIZE PRICE
IVD5116 HiPure FFPE DNA/RNA Kit 50 preps $298.00

Introduction

The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. The Purified DNA/RNA is used for RT-PCR and PCR detection.

Details


Specifications

Features Specifications
Main FunctionsC Co-isolation DNA and RNA from a single FFPE tissue sample
Applications RT-PCR, cDNA synthesis, PCR and second-generation sequencing, etc.
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type FFPE slice, FFPE embedded tissue
Sample amount No more than six 10µm sections of 150mm2 surface area or three 20µm sections of 150mm2 surface area.


Principle

FFPE samples are incubated in an optimized lysis buffer, which results in the release of RNA and precipitation of DNA. After centrifugation, the RNA-containing supernatant and DNA-containing pellet are then processed separately to purify RNA and DNA. For RNA purification, transfer RNA Lysate to an adsorption column and RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, RNA was finally eluted with low-salt buffer. For DNA purification, transfer DNA Lysate to an adsorption column and DNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, DNA was finally eluted with low-salt buffer.

Advantages

1.Use non-toxic dewaxing solution without contact with xylene 
2.Obtain both DNA and RNA simultaneously from the same sample. Elute separately without affecting each other (Have the same steps and effects as top brand 80234, perfect substitute.)

Kit Contents

Contents IVD5116
Purification Times 50 Preps
HiPure DNA Micro Column 50
HiPure RNA Mini Column I 50
2ml Collection Tubes
150
Proteinase K
50 mg
Protease Dissolve Buffer 5 ml
Buffer DPS
60 ml
Buffer FRL
15 ml
Buffer ATL 15 ml
Buffer RLC 15 ml
Buffer AL
15 ml
Buffer VHB
44 ml
Buffer RW2
25 ml
RNase Free Water 10 ml
Buffer AE 10 ml


Storage and Stability

Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.


Experiment Data


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