MagPure A4 XP

MagPure A4 XP utilizes Magen’s solid-phase paramagnetic bead technology for high-throughput purification of PCR amplicons. AmPure utilizes an optimized buffer to selectively bind PCR amplicons 100bp and larger to paramagnetic beads. Excess primes, nucleotides, salts and enzymes can be removed using a simple washing procedure. The resulting purified PCR product is essentially free of contaminants.

Specifications

Principle

This product is based on the purification method of high binding magnetic particles. PCR amplicons mix with MagPure A3, 100bp and larger DNA binds to magnetic beads. Excess primes, nucleotides, salts and enzymes can be removed using a simple washing procedure and finally DNA was eluted by Elution Buffer or Water.

Technical Validation

MagPure A4 XP was evaluated using internal quality-control testing for DNA fragment size selection, microscale DNA marker recovery and background nucleic acid assessment. The tested A4XP batch was compared with a standard control batch across multiple bead-to-sample ratios.

In macro-scale DNA marker size selection testing, MagPure A4 XP showed ratio-dependent fragment recovery patterns from 0.6× to 1.8× bead volume. Agarose gel analysis showed no obvious difference between the tested batch and the control batch, supporting consistent size selection behavior across the tested ratios.

Microscale DNA marker recovery was further evaluated at 0.6×, 0.9×, 1.2× and 1.8× bead ratios. The tested batch showed average recovery rates of approximately 30.2%, 58.4%, 70.7% and 86.9%, respectively. Recovery at 0.9×, 1.2× and 1.8× was comparable to the control batch, while the lower recovery observed at 0.6× was attributed to small elution-volume variation under the 50 µL elution condition.

Background nucleic acid testing showed Qubit readings below the detectable range for the tested A4XP bead batch, indicating no detectable nucleic acid background under the tested conditions.

In addition, MagPure A4 XP was evaluated against AMPure XP in library preparation cleanup and size selection workflows using pooled cfDNA samples. In single size selection testing, the tested MagPure A4 XP batches produced mean library concentrations of approximately 7.99–8.32 ng/µL, compared with 6.35 ng/µL for AMPure XP under the tested conditions.

In double size selection testing, MagPure A4 XP batches produced mean library concentrations of approximately 3.15–3.72 ng/µL, comparable to 3.39 ng/µL for AMPure XP. Microscale 50 bp DNA marker recovery also showed comparable high recovery at 1.2× and 1.5× bead ratios, with MagPure A4 XP batches reaching approximately 93–97% recovery.

These results support the use of MagPure A4 XP for PCR product purification, library cleanup and NGS size selection workflows requiring consistent bead performance, controlled fragment recovery and AMPure XP-like workflow compatibility.

Kit Contents

Storage and Stability

MagPure A4 XP should be stored at 2-8°C upon arrival and is stable up to 18 months under the condition. However, short-term storage (up to 4 weeks) at room temperature (15-25°C) does not affect its performance. — Shake the reagent well before use. It should appear homogenous and consistent in color.

DO NOT FREEZE.