Introduction
Lysozyme is an alkaline enzyme that can hydrolyze mucopolysaccharides in bacteria. Lysozyme mainly destroys the β- 1,4 glycosidic bonds between N-acetyl cell wall acid and N-acetylglucosamine, decomposing insoluble mucopolysaccharides in the cell wall into soluble glycopeptides, which results in the rupture of the cell wall and the escape of contents, leading to the dissolution of bacteria. Lysozymes can also directly bind to negatively charged viral proteins, forming complexes with DNA, RNA, and apo proteins to deactivate the virus. This enzyme widely exists in various tissues of human body, as well as egg whites of birds and poultry, tears, saliva, plasma, milk and other liquids of mammals, microorganisms, of which the highest content is in egg white.
For microbial samples requiring enzymatic pretreatment before nucleic acid purification, explore Magen microbial and pathogen extraction systems for PCR and sequencing workflows.
Details
Specifications
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Features
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Specifications
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CAS No.
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12650-88-3
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Molecular Weight
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14300
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Appearance
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White lyophilized powder
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Purity
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Molecular biology level
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Specific activity
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≥20000U/mg
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Temperature characteristic
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37°C
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PH characteristics
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5.3-6.4
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Preservation Conditions
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It is recommended to store at -20°C to ensure the stability of activity to the greatest extent (normal temperature transportation or storage will not reduce enzyme activity).
The shelf life is up to 3 years at -20°C and 1 year at 2~8°C.
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Usage method
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Bacterial RNA/DNA extraction:
1. Take 1-3ml culture medium in the exponential growth phase, centrifuge at 10,000 x g for 1 minute to collect bacteria, remove the culture medium.
2. Add 100-250μl buffer TE to resuspend bacteria, add 10μl Lsyzozyme (100mg/ml), invert and mix well.
3. Incubate at 25-37°C for 5-30 minutes.
4. Centrifuge at 10,000 x g for 1 minute to collect bacterial protoplasts, discard the liquid, then extract DNA or RNA by other procedures.
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Preparation method
100mg/tube: Add 1ml Buffer PDB to the bottle, mix by inverting, and store at -20°C.
1g/bottle: Add 10ml Buffer PDB to the bottle, mix by inverting, and store in separate packages at -20°C.
Note: Dissolved solution: 50% glycerol, 20mm Tris, pH 7.4, 10mm CaCl2, 0.1% preservative
Ordering information
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Cat No.
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Size
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Preservation Conditions
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Note
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C12136
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100mg/tube
(1.5ml Screw tube）
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This product is transported at room temperature and stored at -20-8°C after receive.
After dissolution, it should be stored at -20°C.
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with 1.5ml Buffer PDB
with 30ml Buffer STE
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C12137
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1g/bottle
(15ml Bottle）
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with 15ml Buffer PDB
with 100ml Buffer STE
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