| CAT NO | PRODUCT NAME | SIZE | PRICE |
| D316702 | HiPure SF Plant DNA 96 Kit | 4 x 96 preps | $796.00 |
| D316703 | HiPure SF Plant DNA 96 Kit | 20 x 96 preps | $3,729.00 |
The HiPure SF Plant DNA 96 Kit (D3167) extends the SDS-based plant DNA extraction workflow into a 96-well plate format for parallel processing.
It is designed for high-throughput genotyping and screening workflows using routine plant samples.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from 50mg simple plant using 96 well bind plate |
| Applications | PCR, SSR, AFLP, RAPD and southern blot, etc. |
| Purification method | 96 well plate |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Economic plant samples |
| Sample amount |
Fresh / frozen plant samples: ≤50mg Dried plant / seed samples: ≤15mg |
| Elution volume | ≥75μl |
| Time per run | 30-50 minutes |
| Liquid carrying volume per column | 800μl |
| Binding yield of column | 100μg |
Plantmaterial is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated. Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged. DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.
Kit Contents
| Contents | D316702 | D316703 |
| Purification Times | 4 x 96 Preps | 20 x 96 Preps |
|
RNase Solution |
5 ml |
22 ml |
|
Elution Buffer |
120 ml |
500 ml |
| Buffer SPL | 200 ml | 2 x 500 ml |
| Buffer PS | 100 ml | 400 ml |
|
Buffer GW1 |
220 ml |
5 x 220 ml |
| Buffer GW2 | 100 ml | 3 x 100 ml |
|
1.6ml Collection Plate |
4 |
20 |
|
HiPure gDNA Plate |
4 |
20 |
|
2.2ml Collection Plate(DW Plate) |
8 |
40 |
|
0.8ml Collection Plate(DW Plate) |
4 |
20 |
|
Sealing Film |
20 |
100 |
Storage and Stability
RNase solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
HiPure SF Plant DNA 96 Kit (D3167) is designed for high-throughput DNA extraction from routine plant samples in plate format.
For flexible manual workflows, HiPure SF Plant DNA Kit (D3164) may be used.
For automated workflows, magnetic bead–based systems such as MagPure Plant DNA Kit (D6351) can be considered.
For a complete selection guide:
D3124
R4150
D6359D
R6672C
IVD3102
R4111
R6327
D3126
IVD5435
IVD3182
D3111
