| CAT NO | PRODUCT NAME | SIZE | PRICE |
| D316402 | HiPure SF Plant DNA Kit | 50 preps | $99.00 |
| D316403 | HiPure SF Plant DNA Kit | 250 preps | $447.00 |
The HiPure SF Plant DNA Kit (D3164) is designed for rapid genomic DNA extraction from routine plant tissues with low levels of polysaccharides and polyphenols.
The workflow uses SDS-based lysis combined with silica column purification, enabling fast DNA isolation without organic extraction. It is suitable for standard PCR and genotyping applications.
| Features | Specifications |
| Main Functions | Isolation total DNA from100 mg simple plant |
| Applications | PCR, SSR, AFLP, RAPD and southern blot, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Economic plant samples |
| Sample amount |
Fresh / frozen plant samples: ≤100mg gDried plant / seed samples: ≤20mg |
| Elution volume | ≥30μl |
| Time per run | 30-50 minutes |
| Liquid carrying volume per column | 800μl |
| Binding yield of column | 100μg |
Validation experiments showed:
| Contents | D316402 | D316403 |
| Purification Times | 50 Preps | 250 Preps |
|
RNase A |
10 mg |
50 mg |
|
Protease Dissolve Buffer |
1.8 ml |
5 ml |
| Buffer SPL | 30 ml | 150 ml |
| Buffer PS | 10 ml | 50 ml |
|
Buffer GW1 |
22 ml |
110 ml |
| Buffer GW2* | 12 ml | 2 x 50 ml |
|
Buffer AE |
15 ml |
60 ml |
|
HiPure gDNA Mini Columns |
50 |
2 x 125 |
|
2 ml Collection Tubes |
50 |
2 x 125 |
RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
HiPure SF Plant DNA Kit (D3164) is suitable for routine plant samples with low polysaccharide and polyphenol content.
For inhibitor-rich or difficult plant samples, CTAB-based systems such as HiPure HP Plant DNA Kit (D3187) may provide improved DNA purity.
For high-throughput or automated workflows, magnetic bead–based systems such as MagPure Plant DNA Kit (D6351) can be considered.
For a complete selection guide:
D3124
R4150
D6359D
R6672C
IVD3102
R4111
R6327
D3126
IVD5435
IVD3182
D3111
