MagPure Seed DNA Kit

The MagPure Seed DNA Kit (D6352) is optimized for DNA extraction from seed and storage tissues, which often contain high levels of starch and storage compounds.

The workflow combines detergent-based lysis with magnetic bead purification and optimized impurity control.

Workflow

Workflow Overview

The MagPure Seed DNA Kit uses a wet bead-beating magnetic workflow for purification of genomic DNA from seed samples, young leaf/fruit samples and difficult-to-grind plant or fungal tissue. Samples are disrupted and lysed in Buffer SOL using bead-beating or high-speed homogenization, followed by heat incubation and centrifugation to clarify the lysate. The DNA-containing supernatant is then combined with Buffer MPB and magnetic beads, allowing DNA to bind to the bead surface before magnetic separation, washing, drying and elution.

Sample Handling Logic

This workflow is designed for sample types where dry grinding alone may not provide consistent disruption or where wet homogenization helps simplify sample handling. Seed samples can be processed after powder preparation and wet bead-beating, while young leaf/fruit and difficult-to-grind samples can be directly homogenized with beads in Buffer SOL. For workflows requiring higher-molecular-weight genomic DNA, the kit also provides an alternative route using liquid-nitrogen grinding followed by Buffer SOL and Buffer SDS lysis. After lysate clarification, the downstream magnetic-particle purification follows a consistent bind, wash, dry and elute workflow.

Time and Workflow Characteristics

Under typical manual operation, the workflow is usually completed within about 60–80 minutes, depending mainly on seed grinding, bead-beating efficiency, lysate clarity, magnetic separation and drying control. The workflow is suitable for laboratories that need a magnetic plant DNA extraction route with flexible sample entry options, including seed-focused wet bead-beating and an alternative high-molecular-weight gDNA route. For detailed step-by-step conditions, workflow guidance and estimated processing times, please refer to the Workflow Note in the Download section.

Specifications

Kit Contents

Storage and Stability

MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Experiment Data

MagPure Seed DNA Kit (D6352) is designed for seed samples and storage tissues that may contain high levels of starch or complex components.

For general plant tissues, MagPure Plant DNA Kit (D6351) is recommended.

For inhibitor-rich plant samples, CTAB-based systems such as HiPure HP Plant DNA Kit (D3187) may provide improved performance.

For a complete selection guide:

👉 Plant DNA and RNA Extraction Selection Guide