Introduction
Soil DNA extraction is challenged by complex sample composition, including diverse microbial populations, variable biomass distribution and high levels of co-extracted inhibitors. Direct extraction methods are widely used to maximize recovery of total microbial DNA, but often face difficulties related to cell disruption efficiency, humic acid contamination and inconsistent DNA yield.
HiPure Soil DNA Kit is developed to address these challenges through a combination of mechanical disruption and optimized purification chemistry. The workflow enables effective lysis of bacteria and fungi while reducing co-extracted inhibitors such as humic substances and soluble metal ions, supporting reliable DNA recovery from diverse soil types.
Within the Magen microbial DNA extraction systems, this kit serves as the standard column-based solution for soil microbiome studies. For high-throughput processing, laboratories may refer to HiPure Soil DNA 96 Kit, while automated workflows can be supported using MagPure Soil DNA Kit.
Details
Specifications
|
Features
|
Specifications
|
|
Main Functions
|
Isolation DNA from 200-500mg soil sample
|
|
Applications
|
PCR, southern blot and enzyme digestion, etc.
|
|
Purification method
|
Mini spin column
|
|
Purification technology
|
Silica technology
|
|
Process method
|
Manual (centrifugation or vacuum)
|
|
Sample type
|
Soil
|
Sample amount
|
200-500mg
|
|
Elution volume
|
≥30μl
|
|
Time per run
|
≤60 minutes
|
|
Liquid carrying volume per column
|
800μl
|
|
Binding yield of column
|
100μg
|
Extraction Principle
Soil samples are first subjected to bead-assisted mechanical disruption combined with chemical lysis to release microbial DNA from complex matrices. Following lysis, insoluble debris and inhibitory substances are reduced using an adsorbent-based cleanup step designed to remove humic acids and other contaminants.
Under optimized binding conditions, DNA is selectively adsorbed onto a silica membrane and purified through sequential washing steps to remove residual inhibitors and soluble impurities. Purified genomic DNA is then eluted for downstream molecular analysis.
Engineering Features
Bead-assisted lysis for complex microbial populations
Mechanical disruption combined with chemical lysis supports DNA release from bacteria and fungi present in soil matrices.
Adsorbent-based inhibitor removal
Specialized cleanup chemistry reduces humic substances and environmental contaminants while minimizing nucleic acid loss during purification.
Optimized column purification system
Silica membrane workflow is designed to maintain DNA recovery consistency across soil types with different compositions and inhibitor backgrounds.
Flexible workflow without specialized equipment
Effective lysis can be achieved using standard vortex mixing, supporting routine laboratory operation without dedicated bead-beating instruments.
Validation
Validation across multiple soil types, including forest soil, agricultural soil and contaminated environmental samples, demonstrated consistent DNA recovery and stable purity profiles. Extracted DNA was directly compatible with 16S PCR amplification, indicating effective removal of humic acid and other inhibitory substances.
Additional testing confirmed that the adsorbent-based cleanup process does not introduce measurable DNA loss during inhibitor removal, supporting reliable recovery across variable soil matrices.
Kit Contents
|
Contents
|
D314202
|
D314203
|
|
Purification Times
|
50 Preps
|
250 Preps
|
|
Hipure DNA Mini Columns II
|
50
|
250
|
|
2ml Collection Tubes
|
50
|
250
|
2ml Bead Tubes
|
50
|
250
|
Buffer SOL
|
60 ml
|
250 ml
|
|
Buffer SDS
|
5 ml
|
20 ml
|
Buffer PS
|
10 ml
|
50 ml
|
Absorber Solution
|
10 ml
|
50 ml
|
|
Buffer GWP
|
40 ml
|
220 ml
|
Buffer DW1
|
30 ml
|
150 ml
|
|
Buffer GW2*
|
20 ml
|
2 x 50 ml
|
|
Buffer AE
|
15 ml
|
30 ml
|
Storage and Stability
Absorber Solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
Purchase Guide
For guidance on selecting the most appropriate nucleic acid extraction system based on sample type, input volume and workflow requirements:
👉 Magen Kits Selection Guide
