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Lysozyme

C12136
CAT NO PRODUCT NAME SIZE PRICE
C12136 Lysozyme (with dissolve buffer)) 100 mg $26.00
C12137 Lysozyme (with dissolve buffer)) 1 g $65.00

Introduction

Lysozyme is an alkaline enzyme that can hydrolyze mucopolysaccharides in bacteria. Lysozyme mainly destroys the β- 1,4 glycosidic bonds between N-acetyl cell wall acid and N-acetylglucosamine, decomposing insoluble mucopolysaccharides in the cell wall into soluble glycopeptides, which results in the rupture of the cell wall and the escape of contents, leading to the dissolution of bacteria. Lysozymes can also directly bind to negatively charged viral proteins, forming complexes with DNA, RNA, and apo proteins to deactivate the virus. This enzyme widely exists in various tissues of human body, as well as egg whites of birds and poultry, tears, saliva, plasma, milk and other liquids of mammals, microorganisms, of which the highest content is in egg white.

Details

Specifications

Features Specifications
CAS No. 12650-88-3
Molecular Weight 14300
Appearance White lyophilized powder
Purity Molecular biology level
Specific activity ≥20000U/mg
Temperature characteristic 37°C
PH characteristics
5.3-6.4
Preservation Conditions

It is recommended to store at -20°C to ensure the stability of activity to the greatest extent (normal temperature transportation or storage will not reduce enzyme activity).

The shelf life is up to 3 years at -20°C and 1 year at 2~8°C.

Usage method

Bacterial RNA/DNA extraction:

1. Take 1-3ml culture medium in the exponential growth phase, centrifuge at 10,000 x g for 1 minute to collect bacteria, remove the culture medium.

2. Add 100-250μl buffer TE to resuspend bacteria, add 10μl Lsyzozyme (100mg/ml), invert and mix well.

3. Incubate at 25-37°C for 5-30 minutes.

4. Centrifuge at 10,000 x g for 1 minute to collect bacterial protoplasts, discard the liquid, then extract DNA or RNA by other procedures.


Preparation method

100mg/tube: Add 1ml Buffer PDB to the bottle, mix by inverting, and store at -20°C.

1g/bottle: Add 10ml Buffer PDB to the bottle, mix by inverting, and store in separate packages at -20°C.

Note: Dissolved solution: 50% glycerol, 20mm Tris, pH 7.4, 10mm CaCl2, 0.1% preservative


Ordering information

Cat No. Size
Preservation Conditions Note
C12136

100mg/tube

(1.5ml Screw tube)

This product is transported at room temperature and stored at -20-8°C after receive.

After dissolution, it should be stored at -20°C.

with 1.5ml Buffer PDB

with 30ml Buffer STE

C12137

1g/bottle

(15ml Bottle)

with 15ml Buffer PDB

with 100ml Buffer STE

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