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Your present location:Home/Products/DNA&RNA Purification/Plasmid DNA/Plasmid DNA/High Pure Plasmid/HiPure Plasmid Mini Kit II
  • plasmid DNA mini prep kit for 5 to 15 mL bacterial culture

HiPure Plasmid Mini Kit II

P1002
CAT NO PRODUCT NAME SIZE PRICE
P100202 HiPure Plasmid Mini Kit II 50 preps $60.00
P100203 HiPure Plasmid Mini Kit II 250 preps $273.00

Introduction

HiPure Plasmid Mini Kit II is the larger-input support product within the standard Magen plasmid mini-prep branch. It processes 5–15 mL of bacterial culture and recovers up to 70 µg of routine molecular-biology-grade plasmid DNA. The workflow retains the familiar alkaline lysis and silica membrane purification format while increasing the culture input and recovery capacity available from a single manual mini-prep column.

P1002 is intended for laboratories that need more DNA than a conventional 1–5 mL mini prep can provide without moving directly to midi-scale handling. For most routine mini-prep workflows, HiPure Plasmid Mini Kit (P1001) remains the core standard column product. When culture input increases to 25–50 mL, P1013 provides the corresponding FastFilter midi-scale route.

Details

Kit Contents

Contents P100202 P100203
Purification Times 50 Preps 250 Preps
RNase A 5 mg 20 mg
Buffer P1 30 ml 140 ml
Buffer P2
30 ml
140 ml
Buffer P3
40 ml
200 ml
Buffer PW1
30 ml
140 ml
Buffer PW2* 12 ml 50 ml
Elution Buffer 15 ml 30 ml
HiPure DNA Mini Columns III 50 250 ml
2 ml Collection Tubes 50 250 ml


Storage and Stability

The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A,Buffer P1 is stable for 6 months when stored at 2-8°C.

Purchase Guide

For guidance on selecting between standard and low-endotoxin plasmid workflows based on copy number, culture input and processing format, please refer to the Plasmid DNA Kits Purchase Guide.

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