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HiPure Viral Mini Column C13112

CAT NO PRODUCT NAME SIZE PRICE
C13112 HiPure Viral Mini Column 1000/Bag $320.00

Introduction

Magen’s HiPure columns are prepared by high quality glass fiber filter membrane as raw materials through membrane cutting, membrane release, ring release, ring pressing, gland, weighing and other processes. HiPure nucleic acid adsorption columns have the characteristics of long-term stability and high binding capacity. Experiments show that the highest binding capacity and binding efficiency of HiPure nucleic acid adsorption columns are basically unchanged when stored at room temperature for 4 years.

The series of nucleic acid columns produced by Magen Biotech are based on carefully selected imported glass fiber membranes (GF/B, GF/D, GF/F). Columns production processes such as polypropylene injection molding materials, injection molding process, and downstream membrane packing and compression rings are strictly controlled. This is to ensure that the column has extremely high adsorption capacity and long-term stability. Compared with conventional products on the market, Magen's columns are with varieties, and binding rate will not change when stored at room temperature for 4 years.

Details


Specifications

Features Specifications
Recommended application Small amounts of nucleic acid isolation, viral nucleicacid from cell free samples
Preservation conditions Room temperature
Stability Up to 4 years
Filter membrane High quality glass fiber filter GF/F, 3 layers
Membrane aperture 0.7μm
Maximum binding yield of plasmid 30 μg
Maximum yield of alcohol mediated Binding
200 μg
Single liquid carrying capacity of column
800 μl
Minimum elution volume
30 μl
Withstand centrifugal force
16,000 x g
Centrifuge
Small high speed centrifuge (2ml)


Adsorption Mechanism

Based on the negatively charged DNA skeleton, it has a high affinity for positively charged glass fibers. In high salt and ethanol solutions, DNA/RNA binds to glass fiber and interacts with hydrophilic matrix on silica through hydrogen bond. DNA/RNA is tightly bound. All pollutants can be removed by washing solution. At high salt concentration, nucleic acids selectively bind to silicagel membrane, while other pollutants, mainly proteins, are removed by membrane washing.


Ordering information

CAT.No. Product Name
Package
C13112

HiPure Viral Mini Column I (3 x GF/F)

with 2ml Collection Tubes

1000/Bag

Purchase Guide

Item No. Product Name

Membrane type/number of layers

Collection tubes

Plasmid DNA binding capacity (Physical adsorption)

gDNA/RNA binding capacity (Alcohol-mediated adsorption)

Minimum Elution volume

Liquid volume capacity

C13010
HiPure DNA Nano Column 2 layers GF/F

2ml without cap

g 20μg
10μl 700μl
C13011
HiPure DNA Micro Column 3 layers GF/F

2ml without cap

10μg 50μg
15μl 700μl
C13100
HiPure DNA Mini Column I 2 layers GF/B

2ml without cap

15μg 100μg
30μl 700μl
C13110
HiPure DNA Mini Column II 4 layers GF/B

2ml without cap

35μg 200μg
50μl 800μl
C13111
HiPure RNA Mini Column 3 layers GF/B

2ml without cap

30μg 200μg
30μl 800μl
C13112
HiPure Viral Mini Column 3 layers GF/F

2ml without cap

30μg 200μg
30μl 800μl
C13113
HiPure CFDNA Mini Column 3 layers GF/F,1 layer GF/B

2ml without cap

30μg 200μg
30μl 800μl
C13120
HiPure DNA Midi Column 4 layers GF/B

15ml Centrifuge tube

125μg 1mg
500μl 4ml
C13121
HiPure DNA Midi Column III 8 layers GF/B

15ml Centrifuge tube

250μg 1mg
500μl 4ml
C13122
HiPure DNA Maxi Column 4 layers GF/B

50ml Centrifuge tube

500μg 5mg
1000μl 20ml
C13123
HiPure DNA Maxi Column III 8 layers GF/B

50ml Centrifuge tube

1mg 5mg 1000μl 20ml
C13124
HiPure DNA Maxi Column C 8 layers GF/B

50ml high speed Centrifuge tube

1mg 5mg
700μl 12ml
C13130
HiPure DNA Plate 2 layers GF/F
1.6ml Plate
30μg
100μg
80μl 900μl
C13131
HiPure gDNA Plate 2 layers GF/B
1.6ml Plate
30μg
100μg
80μl 900μl

Note: GF/B pore size is for 1.0μM glass fiber membrane; GF/F pore size is for 0.7μm glass fiber membrane.

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