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HiPure Soil RNA Kit

R418302 HiPure Soil RNA Kit 50 preps $298.00
R418303 HiPure Soil RNA Kit 250 preps $1,343.00


HiPure Soil RNA Kit is suitable for extracting high-purity microbial total RNA from soil samples. The kit adopts silica gel column purification technology and original humic acid adsorbent technology. It is suitable for extracting high-yield and high-purity total RNA from various soil samples, such as forest soil, grassland soil, mining soil, sediment and so on. The obtained RNA can be directly used in RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation.



Features Specifications
Main Functions Isolation total RNA from 500 mg soil sample
Applications RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Forest soil, grassland soil, mining area soil, sediment and other samples
Sample amount 500 mg
Elution volume ≥30μl
Time per run ≤60 minutes
Liquid carrying volume per column 800µl
Binding yield of column 100µg


Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.


  • High quality - high purity total RNA can be directly used in various sensitive downstream applications
  • Fast - extraction of several samples can be completed in 60 minutes by column purification method
  • Safe - no phenol chloroform extraction required
  • Sensitive - RNA can be purified at the level of PG

Kit Contents

Contents R418302 R418303
Purification Times 50 Preps 250 Preps
HiPure RNA Micro Columns 50 250
2ml Collection Tubes
gDNA Filter Column
2ml Beads Tubes 50 250
Buffer SOL
30 ml
150 ml
Buffer SDS
4 ml
15 ml
Buffer PHC
30 ml
150 ml
Buffer GDP
40 ml
150 ml
Buffer RW1
50 ml
200 ml
Buffer RW2 *
20 ml
2 x 50 ml
RNase Free Water
10 ml
30 ml

Storage and Stability

Buffer PHC should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under theseconditions.The entire kit can be stored at 2–8°C, but in this case buffers should be redissolvedbefore use. Make sure that all buffers are at room temperature when used.

Experiment Data


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