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HiPure Stool DNA Mini Kit

D3141F
CAT NO PRODUCT NAME SIZE PRICE
D314102F HiPure Stool DNA Mini Kit 50 preps $174.00
D314103F HiPure Stool DNA Mini Kit 250 preps $746.00

Introduction

This kit combines bead grinding and column purification techniques and is suitable for rapid extraction of high-purity total DNA from ≤200mg stool samples. The purification column selects high-performance ultrafine glass fiber filter membrane as the material, which efficiently and specifically adsorbs DNA. This kit adopts silica gel column purification technology and a unique salt mediated adsorption method, which can efficiently remove RNA and inhibitory factors. The entire process does not require phenol chloroform and can efficiently adsorb humic acid and other inhibitory factors in the solution. Purified DNA can be directly used for experiments such as PCR, Southern hybridization, enzyme digestion, and second-generation sequencing.

Details

Kit Contents

Contents D314102F D314103F
Purification Times 50 Preps 250 Preps
HiPure DNA Mini Columns II 50 250
2ml Collection Tubes 50 250
2ml Bead Tubes
50
250
Proteinase K
24 mg
120 mg
Protease Dissolve Buffer 1.8 ml 10 ml
Buffer STL
40 ml
180 ml
Buffer PCI
20 ml
90 ml
Buffer SL
5 ml
25 ml
Buffer GWP 70 ml 2 x 170 ml
Buffer GW2* 20 ml 2 x 50  ml
Elution Buffer 15 ml 60 ml


Storage and Stability

Proteinase K and Buffer PCI should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

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