Your present location:Home/Products/DNA&RNA Purification/RNA/Tissue/Cell RNA/HiPure Total RNA Kit
  • Substitute Qiagen 74104.png

HiPure Total RNA Kit


R401102 HiPure Total RNA Kit 50 preps $99.00
R401103 HiPure Total RNA Kit 250 preps $448.00


The HiPure Total RNA Kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.



Features Specifications
Main Functions Isolation total RNA (not include miRNA) from animal tissues, cells and simple plant tissues using one column
Applications RT-PCR, qRT-PCR, Northern hybridization, second generation sequencing
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Animal soft tissue, cultured cells, lymphocytes, simple plant tissue
Sample amount

Cells: 1 x 107

Animal tissue: 1-20 mg

Plant leaves: 50-150 mg

Yeast cells: 5 x 106

Yield 2-100μg
Elution volume 50μl
Time per run 15 minutes(1-24 samples
Liquid carrying volume per column 800µl
Binding yield of column 100µg


HiPure RNA technology simplifies total RNA isolation. Samples are first lysed and then homogenized. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the HiPure silica membrane and RNA binds to the silica membrane, and all contaminants are efficiently washed away. For certain RNA applications that are sensitive to very small amounts of DNA, the residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment. Pure, concentrated RNA is eluted in water.


  • Efficient removal of DNA - unique genomic DNA removal column without DNase treatment
  • High quality - high purity total RNA can be directly used in various sensitive downstream applications
  • Fast - several samples can be extracted in 20 minutes by column method
  • Safe - no phenol chloroform extraction required
  • Sensitive - RNA can be recovered at the level of PG

Kit Contents

Contents R401102 R401103
Purification Times 50 Preps 250 Preps
HiPure RNA Mini Columns 50 250
2ml Collection Tubes 50 250
RTL Lysis Buffer
50 ml
200 ml
RNA Binding Buffer
15 ml
75 ml
Buffer RW1 50 ml 200 ml
Buffer RW2*
20 ml
2 x 50 ml
RNase Free Water
10 ml
30 ml

Storage and Stability

The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.


Leave A Message

Leave A Message

    Please provide us with the information below, and we'll contact you as soon as possible.

This site uses cookies to improve your online experience,allow you to share content on social media,measure traffic to this website and display customised ads based on your browsing activity.