HiPure DNA/RNA Kit

This Kit is designed to purify genomic DNA and total RNA simultaneously from a single biological sample. Lysate is first passed through a DNA spin column to selectively isolate DNA and then through an RNA column to selectively isolate RNA. Pure DNA and RNA are purified from the entire sample, in contrast to other procedures where either the biological sample or the purified total nucleic acids is divided into two before being processed separately. The kit is compatible with small amounts of a wide range of animal cells and tissues.

Specifications

Principle

The Kits are designed to purify both genomic DNA and total RNA from the same cellor tissue sample. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column and bind DNA. Ethanol is added to the flow-through and the sample is applied to an RNA column. DNA/RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit. High-quality DNA is eluted in as little as 50µl water using the Kit.

Technical Validation

HiPure DNA/RNA Kit was evaluated as a column-based co-isolation workflow for sequential purification of genomic DNA and total RNA from the same biological sample. In this workflow, the lysate is first passed through a DNA spin column to capture genomic DNA, while the flow-through is subsequently adjusted and applied to an RNA column for total RNA purification. This design avoids splitting the original sample before extraction and supports paired DNA and RNA analysis from limited tissue or cell inputs.

Co-isolation performance was tested using 10 mg chicken tissue inputs, including liver, kidney, spleen and lung. From 10 mg chicken liver input, the workflow produced total RNA yields of 41.0–43.8 µg, with A260/280 values of 2.10–2.11 and A260/230 values of 1.72–1.98. The corresponding genomic DNA yields were 13.7–14.93 µg, with A260/280 values of 1.96. Kidney, spleen and lung samples also produced measurable paired RNA and DNA fractions under the tested conditions. Electrophoresis analysis showed clear RNA band patterns and intact genomic DNA bands, supporting simultaneous recovery of both nucleic acid types from the same sample.

A comparison with a reference commercial DNA/RNA co-isolation workflow was performed using chicken liver inputs of 40 mg, 20 mg, 10 mg and 5 mg. Across the tested input range, R5111 produced RNA yields of 156.08–147.03 µg from 40 mg liver input, 98.47–94.70 µg from 20 mg input, 41.29–46.58 µg from 10 mg input and 21.04–18.64 µg from 5 mg input. The corresponding DNA yields were 52.68–53.18 µg, 25.62–24.37 µg, 16.97–16.81 µg and 6.99–8.29 µg, respectively. These results showed recovery in a similar range to the reference workflow under the tested conditions, with consistent A260/280 values for both RNA and DNA fractions.

Downstream compatibility was supported by electrophoresis and PCR analysis. RNA electrophoresis showed visible RNA bands across different liver input amounts, while genomic DNA electrophoresis showed high-molecular-weight DNA bands without obvious smearing. PCR amplification from the purified genomic DNA produced clear target bands across the tested input range, indicating that the DNA fraction was compatible with downstream PCR analysis. Together, these data support R5111 as a practical co-isolation workflow for paired genomic DNA and total RNA recovery from limited animal tissue samples.

Kit Contents

Storage and Stability

HiPure DNA/RNA Kit can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

For guidance on selecting the most appropriate DNA extraction system based on sample type, input volume and workflow requirements:

👉 Magen Kits Selection Guide