Introduction
Stool samples are increasingly used for non-invasive analysis of microbial gene expression and intestinal biology. Compared to DNA extraction, RNA preparation from stool samples presents additional challenges due to RNA instability, partial degradation and the presence of a wide range of inhibitory substances.
Stool samples typically contain polysaccharides, bile salts and other digestion-derived compounds that can interfere with RNA purification and downstream enzymatic reactions. At the same time, RNA molecules are more susceptible to degradation during sample processing, making both inhibitor control and RNA preservation critical for successful extraction.
The HiPure Stool RNA Kit is developed to address these challenges through a workflow combining mechanical disruption with optimized purification chemistry. The system is designed to reduce inhibitor background while maintaining RNA integrity during extraction from complex fecal matrices.
Within the Magen microbial RNA extraction systems, this kit serves as the standard column-based solution for stool RNA preparation. For automated workflows, laboratories may refer to MagPure Stool RNA Kit, while DNA-based analysis can be supported using HiPure Stool DNA mini Kit.
Details
Specifications
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Features
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Specifications
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Main Functions
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Isolation total RNA from 100-150mg stool sample
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Applications
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RT-PCR, Northern hybridization and other experiments
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Purification method
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Mini spin column
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Purification technology
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Silica technology
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Process method
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Manual (centrifugation or vacuum)
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Sample type
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Stool
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Sample amount
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100-150 mg
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Elution volume
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≥30μl
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Time per run
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≤50 minutes
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Liquid carrying volume per column
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100µg
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Binding yield of column
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800µl
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Extraction Principle
Stool samples are first subjected to bead-assisted mechanical disruption combined with chemical lysis to release total RNA from microbial cells within complex fecal matrices.
Following lysis, phase separation is performed using organic extraction to remove genomic DNA and impurities from the lysate. The resulting aqueous phase containing RNA is then subjected to purification.
Under optimized binding conditions, RNA is selectively adsorbed onto a silica membrane and purified through sequential washing steps to remove residual contaminants and inhibitors. Purified RNA is then eluted for downstream molecular analysis.
Engineering Features
RNA stability-focused workflow design
Purification conditions are optimized to preserve RNA integrity during extraction from inhibitor-rich stool samples
Targeted inhibitor control
Workflow reduces bile salts, polysaccharides and digestion-derived contaminants to support reliable downstream RNA analysis
Bead-assisted lysis for microbial RNA release
Mechanical disruption supports RNA recovery from diverse microbial populations
Column-based purification consistency
Silica membrane purification supports reproducible RNA recovery across variable stool samples
Validation
RNA extracted from stool samples demonstrated stable purity profiles and intact electrophoretic patterns without detectable degradation. The purified RNA was compatible with downstream RT-PCR analysis without additional cleanup, indicating effective control of inhibitory substances and preservation of RNA integrity.
Kit Contents
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Contents
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R418502
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R418503
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Purification Times
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50 Preps
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250 Preps
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HiPure RNA Mini Columns
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50
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250
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2ml Collection Tubes
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50
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250
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Glass Beads (0.1~0.6mm)
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30 g
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150 g
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Buffer SPL
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30 ml
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140 ml
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Buffer PHC
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30 ml
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140 ml
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Buffer GRP
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60 ml
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250 ml
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Buffer RW1
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50 ml
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250 ml
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Buffer RW2 *
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20 ml
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2 x 50 ml
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RNase Free Water
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15 ml
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30 ml
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Storage and Stability
The kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions. At low temperatures, Buffer SPL may form precipitates, dissolve it by 55°C water bath. After receiving the product, Buffer PHC should be stored at 2-8°C.
Purchase Guide
For guidance on selecting the most appropriate nucleic acid extraction system based on sample type, input volume and workflow requirements:
👉 Magen Kits Selection Guide
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