Introduction
Magen DNase I is a lyophilized bovine pancreatic deoxyribonuclease designed for removing DNA contamination from protein samples and eliminating background DNA contamination. As an endonuclease, DNase I hydrolyzes phosphodiester bonds in DNA to produce polynucleotides with free 3′-hydroxyl and 5′-phosphate termini.
The enzyme exhibits optimal activity at approximately pH 7.8, is activated by divalent metal ions and is inhibited by chelating agents such as EDTA. Calcium ions can help stabilize the enzyme against degradation by other hydrolases. The product is purified from bovine pancreas by chromatography to reduce contamination from other hydrolytic enzymes. It is not intended for RNA extraction workflows.