The SolPure Blood DNA Kit provides a salt precipitation workflow for purification of high-molecular-weight genomic DNA from whole blood and related biological fluids. The protocol uses detergent-mediated cell lysis followed by protein precipitation and alcohol-based DNA recovery, producing genomic DNA fragments that can exceed 200 kb in size.

The scalable purification workflow allows preparation of genomic DNA suitable for PCR analysis, sequencing preparation, or DNA archiving from routine blood samples.

For silica membrane column purification laboratories may use the HiPure Blood DNA Mini Kit (D3111), while automated extraction workflows are supported by the MagPure Blood DNA Kit (D6311) and MagPure Universal DNA Kit (IVD3102).

Specifications

Principles

Cells are lysed with ananionic detergent in the presence of a DNA stabilizer. The DNA stabilizer limits the activity of intracellular DNases and also DNases found elsewhere in the environment. RNA is then removed by treatment with an RNA digesting enzyme. Other contaminants, such as proteins, are removed by salt precipitation. Finally, the genomic DNA is recovered by precipitation with alcohol and dissolved in Buffer TE. Purified DNA typically has an A260/A280 ratio between 1.7 and 1.9, and is up to 200 kb in size. The DNA can be safely stored at 2-8°C, -20°C, or -80°C.

• Safe - without phenol chloroform extraction
• Environment friendly - the reagents used are safe, non-toxic and without pollution
• High molecular weight - the molecular weight of genomic DNA is about 50-150kb
• High purity - the purified DNA has A260/280=1.8-1.9, A260/230=2.0-2.5
• Unlimited sample size - solution type operation, sample volume can be adjusted at will
• Cost performance - the most economical nucleic acid extraction technology

Storage and Stability

RNase Solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

For guidance on selecting the most appropriate DNA extraction system based on sample type, input volume and workflow requirements:

👉 Magen Kits Selection Guide