Introduction

The HiPure Insect DNA 96 Kit provides a high-throughput workflow for purification of insect genomic DNA using a silica membrane 96-well plate format. The system allows simultaneous processing of multiple insect samples while maintaining consistent purification performance across large sample batches.

The workflow is suitable for laboratories performing insect population genetics studies, molecular identification projects, or large-scale ecological DNA screening.

For individual sample processing laboratories may use the HiPure Insect DNA Kit (D3129), while universal biological samples can be processed using the HiPure Universal DNA Kit (D3018) or MagPure Universal DNA Kit (IVD3102).

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from insect tissue
Applications	PCR, southern bolt and virus detection, etc
Purification method	96 well DNA plate
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Insect tissue samples
Sample amount
Elution volume
Time per run
Liquid carrying volume per column
Binding yield of column

Principles

This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

High purity DNA - can be used in sensitive downstream applications such as multiplex and quantitative pcr
Good repeatability - suitable for extracting high-yield DNA from insect tissue samples
Safe - without phenol chloroform extraction and alcohol precipitation

Kit Contents

Contents	D313901	D313902
Purification Times	1 x 96	4 x 96
HiPure DNA Plate	1	4
2.2 ml Collection Plate	1	4
1.6 ml Collection Plate	1	4
0.5ml Collection Plate	1	4
Seal Film	8	32
Buffer ITL	30 ml	120 ml
Buffer IL	30 ml	125 ml
Buffer GW1	44 ml	2 x 110 ml
Buffer GW2	50 ml	3 x 50 ml
Proteinase K	50 mg	200 mg
Protease Dissolve Buffer	6 ml	15 ml
Buffer AE	20 ml	60 ml

Storage and Stability

Proteinase K, RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in thiscase buffers should be redissolved before use. Make sure that all buffers areat room temperature when used.

Purchase Guide

For guidance on selecting the most appropriate DNA extraction system based on sample type, input volume and workflow requirements:

👉 Magen Kits Selection Guide

Articles

Download

D3139 HiPure Insect DNA 96 Kit.pdf Preview Down

CAT NO	PRODUCT NAME	SIZE	PRICE
D313901	HiPure Insect DNA 96 Kit	1 x 96 Preps
D313902	HiPure Insect DNA 96 Kit	4 x 96 Preps

HiPure Insect DNA 96 Kit

Introduction

Details

Advantages

Kit Contents

Storage and Stability

Purchase Guide

Articles

Download

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