Introduction
The MagPure Forensic DNA Kit (D6359D) is designed for purification of genomic DNA from trace and challenging forensic samples using magnetic bead technology. Forensic evidence often contains extremely limited amounts of DNA and may be partially degraded or contaminated by environmental inhibitors, making reliable DNA recovery critical for downstream genetic analysis.
The kit supports extraction of genomic DNA from a wide range of forensic materials including hair roots, bone fragments, fingernails, blood stains, semen stains and buccal swabs. The magnetic bead workflow is compatible with automated nucleic acid extraction instruments and supports high-throughput forensic DNA purification.
Within the Magen DNA extraction portfolio, the MagPure Forensic DNA Kit serves as a core solution for trace DNA purification and forensic genetic analysis workflows.
Details
Extraction Principle
Forensic samples such as hair, nails and bone often contain highly cross-linked proteins and extremely limited DNA quantities. Efficient DNA extraction therefore requires intensive enzymatic digestion and optimized adsorption chemistry.
In the MagPure Forensic DNA workflow, samples are digested using Proteinase K together with reducing agents such as DTT. Proteinase K breaks down structural proteins and histones, while DTT reduces disulfide bonds in keratinized tissues, enabling efficient release of DNA from highly cross-linked matrices. Extended incubation allows gradual digestion of difficult materials and improves recovery of trace DNA.
Following digestion, DNA molecules bind to magnetic silica particles under chaotropic conditions. The high surface area of the magnetic particles enables efficient capture of DNA even at very low concentrations. Impurities and PCR inhibitors are removed during washing steps, and purified DNA is eluted in a small volume of buffer suitable for downstream PCR amplification and STR analysis.
Engineering Characteristics
Magnetic bead DNA adsorption
High-affinity magnetic particles provide efficient adsorption of genomic DNA under optimized binding conditions, allowing effective enrichment of DNA molecules from dilute forensic lysates.
Trace DNA recovery
The extraction chemistry and binding buffers are optimized for recovery of low-template DNA commonly encountered in forensic samples.
Compatibility with degraded samples
The purification workflow supports recovery of fragmented DNA while minimizing additional degradation during the extraction process.
Automation-ready workflow
The system is compatible with automated nucleic acid extraction instruments and multi-channel purification platforms used in forensic laboratories.
Reduced sample handling
Magnetic separation eliminates repeated centrifugation steps, reducing manual handling and improving workflow reproducibility.
Technical Validation
Internal validation experiments demonstrated stable DNA recovery from challenging forensic sample types including trace biological materials and degraded samples. The magnetic bead purification workflow enables efficient capture of genomic DNA while removing contaminants that may interfere with downstream PCR amplification.
Purified DNA obtained using the MagPure Forensic DNA Kit supports reliable amplification in PCR-based genotyping assays commonly used in forensic laboratories.
Comparative evaluations performed with commonly used forensic DNA purification workflows showed comparable or improved DNA recovery from trace samples, supporting reliable performance for forensic DNA analysis and identification workflows.
Typical Applications
Genomic DNA purified using the MagPure Forensic DNA Kit is commonly used in forensic and trace DNA analysis workflows, including:
• Forensic DNA identification
• Trace DNA purification from biological evidence
• DNA recovery from hair roots and fingernails
• Blood stain and semen stain DNA extraction
• PCR-based genotyping analysis
• STR profiling workflows
• Human identification studies
• Forensic genetics research
Related Products
Forensic laboratories often process a wide range of biological samples in addition to trace evidence. The following nucleic acid extraction systems are frequently used together with the MagPure Forensic DNA Kit.
• HiPure Bone DNA Kit (D3124) – genomic DNA purification from bone fragments used in forensic identification and anthropological studies
• HiPure Universal DNA Kit (D3018) – genomic DNA purification from mixed biological samples including tissue, blood and body fluids
• MagPure Universal DNA Kit (IVD3102) – magnetic bead purification of genomic DNA from diverse biological samples using automated extraction instruments
Kit Contents
|
Contents
|
D635901D
|
D635902D
|
D635903D
|
|
Purification Times
|
48
|
96
|
480
|
|
MagPure Particles N
|
1.1 ml
|
2.2 ml
|
11 ml
|
|
Buffer ATL
|
40 ml
|
80 ml
|
2 x 200 ml
|
|
Buffer BGL
|
40 ml
|
80 ml
|
2 x 200 ml
|
|
Buffer BST1
|
60 ml
|
120 ml
|
550 ml
|
|
Buffer GW2*
|
20 ml
|
25 ml
|
2 x 50 ml
|
|
Proteinase K
|
50 mg
|
120 mg
|
2 x 220 mg
|
|
Protease Dissolve Buffer
|
5 ml
|
10 ml
|
30 ml
|
|
DTT
|
235 mg
|
235 mg
|
3 x 235 mg
|
|
Elution Buffer
|
10 ml
|
10 ml
|
30 ml
|
Prefilled Kit Contents
32/48 channel machine
|
Product
|
Contents and volume
|
D6359D-TL-06
|
|
Buffer BGL
|
|
80 ml
|
|
Buffer ATL
|
|
80 ml
|
|
Proteinase K
|
|
120 mg
|
|
Protease Dissolve Buffer
|
|
10 ml
|
|
DTT Powder
|
|
2 x 235 mg
|
|
Elution Buffer
|
|
5 ml
|
|
AS-Tip
|
|
12 pcs
|
2.0ml V
bottom plate
|
Row 1/7：empty
|
6 plates
|
Row 2/8：500μl Buffer BST1
|
Row 3/9：500μl Buffer BST1
|
Row 4/10：20μl MagPure Particles N
500μl Buffer GW2
Row 5/11：500μl Buffer GW2 (ethanol ~350ul)
|
Row 6/12：60μl Elution Buffer
|
96 channel machine
|
Product
|
Contents and volume
|
D6359D-F-96
|
D6359D-F-48
|
|
Buffer BGL
|
|
80 ml
|
40 ml
|
|
Buffer ATL
|
|
80 ml
|
40 ml
|
|
Proteinase K
|
|
120 mg
|
50 mg
|
|
Protease Dissolve Buffer
|
|
10 ml
|
5 ml
|
|
DTT Powder
|
|
235 mg
|
235 mg
|
|
Elution Buffer
|
|
5 ml
|
5 ml
|
|
96-Tip (AS)
|
|
1
|
1
|
Sample Plate 1
|
500μl Buffer BST1
|
1
|
1
|
Sample Plate 2
|
500μl Buffer BST1
|
1
|
1
|
Wash Plate 1
|
20μl MagPure Particles N
500μl Buffer GW2
|
1
|
1
|
Wash Plate 2
|
500μl Buffer GW2
|
1
|
1
|
Elute Plate
|
60μl Elution Buffer
|
1
|
1
|
Storage and Stability
Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. DTT Powder is easily oxidized, should be shipped at -20~8°C and stored at -20°C upon arrival, keep dry. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these condition.
