DNase Set

Bovine pancreatic deoxyribonuclease (also known as DNase) is an endonuclease. It acts on the phospholipid bond, especially the bond adjacent to pyrimidine nucleoside, to produce polynucleotides with free hydroxyl at the 3 'end and phosphate at the 5' end. The optimum pH value of is 7.8. DNase can be activated by divalent metals and inhibited by chelates such as EDTA and sodium dodecyl sulfate. 5mM calcium ion can be used as a stabilizer to protect DNase from being decomposed by hydrolase. This product is extracted from bovine pancreas and prepared by chromatography to remove the pollution of other hydrolases.

RNase Free DNase I Set is specially designed for column/magnetic bead RNA extraction kit. Biological samples were lysed, ethanol was added to adjust the binding conditions, and transferred to column or magnetic beads to adsorb RNA. After washing, add DNase I and DNase Buffer to the membrane of the column or magnetic bead, digest at room temperature (25-37℃) for 15 minutes to completely remove the DNA adsorbed on the membrane/beads, after wash away DNase and degraded DNA, and finally remove RNA with DEPC water.

For integrated on-column or magnetic bead DNase treatment, explore Magen RNA extraction systems and workflow resources designed for DNA-reduced RNA purification.

Specifications

Advantages

• Pure natural protein, from bovine pancreas
• High activity and purity, >10 kunitz units/μl
• High cost performance
• Completely remove RNase contamination
• Ready to use liquid type
• Ultra high dosage (10μl/time,100μ/time) to remove more genomic DNA

Technical Validation

The DNase I Set was validated for RNase-free performance using bacterial RNA samples. After DNase I treatment, agarose gel electrophoresis showed intact RNA bands without visible RNA degradation, indicating no detectable RNase carryover under the tested conditions.

DNase activity was evaluated using liver tissue DNA extraction samples. Compared with the no-DNase and DNase Buffer control groups, samples treated with DNase I showed a strong reduction in DNA yield by NanoDrop measurement and no obvious genomic DNA band by agarose gel electrophoresis, supporting effective DNA digestion activity.

The effect of DNase I digestion on downstream qPCR detection was further tested using porcine blood samples spiked with HBV DNA. Control samples without DNase treatment showed Ct values around 30, while DNase-treated samples showed no Ct signal, indicating that DNA templates were effectively degraded and no detectable amplifiable DNA remained under the tested conditions.

Stability testing showed that liquid DNase I stored at room temperature for two weeks performed similarly to DNase I stored at 4°C in bacterial RNA extraction and tissue DNA digestion tests. These results support the use of the DNase I Set for on-column or magnetic bead-based RNA extraction workflows requiring reliable genomic DNA removal.

Ordering information

Storage and Stability