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MagPure Particles N C1411

CAT NO PRODUCT NAME SIZE PRICE
C14110 MagPure Particles N 100 ml $599.00
C14111 MagPure Particles N 400 ml $2,199.00
C14112
MagPure Particles N
3 x 400 ml
$5,999.00
C14113
MagPure Particles N
10 x 400 ml
$15,999.00

Introduction

Magnetic bead nucleic acid purification technology uses nano or micron superparamagnetic material as the matrix, generally black ferric oxide or yellowish brown ferricoxide as the magnetic material. The surface of bead is coated with appropriate functional groups, which can adsorb nucleic acid. Magnetic beads commonly used for nucleic acids, containing carboxyl groups, hydroxyl groups, or silicon groups. Silicon-based magnetic beads are the most common, and its principle of adsorbing nucleic acid is consistent with the classical glass milk purification technology or glass fiber filter membrane purification method. Magpure particle is a kind of polydisperse fast speed silica magnetic beads. The core is ferricoxide, accounting for 50%, and the surface coating is silica, accounting for 50%. The product can be used for plasmid extraction, gel DNA recovery, product purification, genomic DNA and RNA extraction, and viral nucleic acid extraction.

Details


Specifications

Features Specifications
Concentration 70 mg/ml
Appearance Suspension of yellowish brown particles
Surface functional group Si-OH, Silanol
Dispersibility Polydisperse Amorphous
Particle size 0.2-2 μm
Preservation conditions

Room Temperature, valid for up to 2 years.

It is recommended to store in 2-8°C to prevent microbial growth.

Magnetic response speed
~60 seconds
Settling velocity
>10 minutes
High salt mediated binding
>2M guanidine isothiocyanate, DNA recovery up to 80%
Alcohol mediated binding
2M guanidine hydrochloride / isopropanol (30%), and the recovery of DNA / RNA was as high as 85%
PEG8000 mediated binding
The recovery of DNA/RNA was up to 85%
DNase/RNase
Not detected
DNA residue
<1 ppm
Recommended application
Plasmid extraction, gel DNA recovery, viral nucleic acid isolation


Principle

Highsalt mediated binding: in the solution containing 2-4M guanidine isothiocyanate, Magpure particles can selectively recover DNA molecules, and impurities such as protein polysaccharides are not adsorbed.

Alcohol mediated binding: in the solution containing guanidine salt and alcohol (>25%), Magpure particles can selectively recover DNA/RNA molecules, and proteins and other impurities are not adsorbed.

After biological samples are treated with digestive solution or lysis Buffer, DNA/RNA is released from cells, organelles and protein complexes (ribosomes and nucleosomes) into reagents. After Magpure particles and binding solution are added, DNA/RNA is adsorbed to the surface of Magpure particles to form DNA/RNA bead complex. Under the action of the magnetic field, the magnetic beads are separated and collected, and the impurities such as protein are removed with the waste liquid. After two or three steps of further cleaning, the DNA/RNA magnetic bead complex is resuspended in sterilized water or TE buffer, and the DNA/RNA falls off from the surface of the magnetic beads, so as to achieve the purpose of purification.


Ordering information

CAT.No. Product Name
Package
C14110
MagPure Particles N
100 ml
C14111
MagPure Particles N
400 ml
C14112
MagPure Particles N
3 x 400 ml
C14113
MagPure Particles N
10 x 400 ml

Purchase Guide

Features MagPure Particles

MagPure Particles N

MagPure Particles G

MagPure Particles F

MagBind Particles
Cat.No.
C1410 C1411

C1412

C1414 C1413
Concentration
100mg/ml 70mg/ml

40mg/ml

50mg/ml 10mg/ml
Form
Amorphous and Porous Amorphous and Porous

Porous

Amorphous Nonporous
Surface function
Si-OH, Silica Beads Si-OH, Silica Beads

Si-OH, Silica Beads

Si-OH, Silica Beads COOH, Carboxyl Beads
Dispersion
Polydisperse Polydisperse

Monodisperse

Monodisperse Monodisperse
Particle Size
1.5-5μm 0.2-2μm

1-1.5μm

0.2-1.5μm 0.8-1μm
Color
Black Yellowish Brown

Dark Brown

Dark Brown Yellowish Brown
Magnetic response
15-30s ~60s

~30s

20s 120s
Settling Time (1ml)
>5min >10min

>3min

>3min >2h
Usage (0.2ml Sample)
20μl 20μl

20-30μl

20-30μl 20-30μl
DNA Recover Rate (only 4M GITC)
>80% >80%

>80%

>80% 0
DNA Recover Rate (10% PEG8000/NaCl)
>85% >85%

>85%

>85% >90%
Recommended Use
  • gDNA/RNA Isolation from Blood, Tissue, Plant, Swab, Spots, Stool, Soil and etc.
  • Viral DNA/RNA Isolation
  • Agarose Gel DNA Purification
  • DNA/RNA Isolation from low nucleic acid content samples
  • Plasmid Isolation
  • DNA/RNA Clean Up
  • Circulating DNA Isolation
  • Viral Nucleic acid Isolation
  • gDNA Isolation
  • FFPE DNA/RNA Isolation
  • Plasmid extraction
  • gel DNA recovery
  • genomicDNA/RNA extraction
  • viral nucleic acid extraction
  • Circulating DNA extraction
  • DNA/RNA Clean Up and concentration
  • DNA/RNA Isolation from low nucleic acid content samples
  • Research immuno assays


  • The MagPure magnetic-particle technology combines the speed and efficiency of silica-based DNA purification with the convenient handling of magnetic particles. DNA binds to the silica surface of the magnetic particles in the presence of a chaotropic salt. DNA bound to the particles is then efficiently washed, considerably improving the purity of DNA. High-quality DNA is eluted. The automated purification procedure completely removes enzymes, nucleotides, and other contaminants and inhibitors. Purified DNA is suitable for direct use in downstream applications, such as sequencing and microarray analysis.

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