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HiPure Total RNA Plus Kit R4111

CAT NO PRODUCT NAME SIZE PRICE
R411102 HiPure Total RNA Plus Kit 50 preps $124.00
R411103 HiPure Total RNA Plus Kit 250 preps $547.00

Introduction

This kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Details


Specifications

Features Specifications
Main Functions Isolation total RNA from 20mg tissue, 150mg plant, 5 x 106 cell using two columns (gDNA removed column)
Applications RT-PCR, qRT-PCR, Northern hybridization, second generation sequencing
Purification method Mini spin column
Purification technology Silica technology, DNA filtration technology
Process method Manual (centrifugation or vacuum)
Sample type Animal soft tissue, cultured cells, lymphocytes, simple plant tissue
Sample amount

Cells:1 x 107

Animal tissue sample: 1-20 mg

Plant tissue: 50-150 mg

Yield 2-100μg
Elution volume 50μl
Time per run 25 minutes(1-24 samples
Liquid carrying volume per column 800µl
Binding yield of column 100µg


Principle

The Kit isolates total RNA from up to 107 cells or 20 mg tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 25 mins. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to a RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit.


Advantages

  • Efficient removal of DNA - unique genomic DNA removal column without DNase treatment
  • High quality - high purity total RNA can be directly used in various sensitive downstream applications
  • Fast - several samples can be extracted in 25 minutes by column method
  • Safe - no phenol chloroform extraction required
  • Sensitive - RNA can be recovered at the level of PG


Kit Contents

Contents R411102 D411103
Purification Times 50 Preps 250 Preps
HiPure DNA Mini Columns
50
250
HiPure RNA Mini Columns 50 250
2ml Collection Tubes 100 2 x 250
Buffer RLC
50 ml
200 ml
Buffer RW1 50 ml 200 ml
Buffer RW2*
12 ml
2 x 50 ml
RNase Free Water
10 ml
30 ml


Storage and Stability

HiPureKit can be stored dry at room temperature (15-25°C) and are stable for at least18 months under these conditions. During shipment, crystals or precipitationmay form in the Buffer RLC. Dissolve by warming buffer to 37°C.

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