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Your present location:Home/Products/DNA&RNA Purification/DNA/Plant DNA/Column Kits/HiPure HP Plant DNA Maxi Kit
  • large scale plant DNA extraction CTAB column workflow.png

HiPure HP Plant DNA Maxi Kit

D3163
CAT NO PRODUCT NAME SIZE PRICE
D316302 HiPure HP Plant DNA Maxi Kit 10 preps $224.00
D316303 HiPure HP Plant DNA Maxi Kit 50 preps $995.00

Introduction

The HiPure HP Plant DNA Maxi Kit (D3163) is designed for large-scale DNA extraction from plant or fungal samples, supporting input up to gram-level tissue.

The workflow is based on CTAB lysis and organic extraction, consistent with D3187, but scaled for bulk DNA preparation.

Details

Specifications

Features Specifications
Main Functions Isolation total DNA from 3g plant and fungal tissue
Applications PCR, SSR, AFLP, RAPD and southern blot, etc.
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Various plant samples (including conventional, polysaccharides and polyphenols)
Sample amount

Fresh / frozen plant samples: 2-3 g

Dried plant / seed samples: 0.5-1 g

Elution volume ≥500μl
Time per run 120 minutes
Liquid carrying volume per column 20ml
Binding yield of column 5mg

Technical Notes

  • Maintains the same inhibitor removal strategy as D3187
  • Includes RNase treatment for improved DNA purity
  • Suitable for workflows requiring higher DNA yield rather than higher throughput
  • Kit Contents

    Contents D316302 D316303
    Purification Times 10 Preps 50 Preps
    RNase A
    20 mg
    90 mg
    Protease Dissolve Buffer
    1.8 ml
    10 ml
    Buffer PAL 180 ml 900 ml
    Buffer GWP 150 ml 800 ml
    Buffer GW2 25 ml 200 ml
    Buffer AE
    30 ml
    120 ml
    HiPure DNA Maxi Columns II
    10 50
    50 ml Collection Tubes
    20 100

    Storage and Stability

    RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

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