MagPure Pathogen DNA/RNA Kit B

MagPure Pathogen DNA/RNA Kit B enables magnetic bead purification of pathogen nucleic acids from biological samples such as plasma, serum and homogenate suspensions. The workflow supports recovery of pathogen DNA and RNA from samples with relatively low cellular background and is compatible with automated extraction platforms commonly used in molecular laboratories.

Following lysis and magnetic bead binding, nucleic acids are purified through sequential washing steps that remove proteins and inhibitory substances before elution. The purified nucleic acids are suitable for sequencing preparation workflows.

Within the Magen pathogen extraction portfolio, MagPure Pathogen DNA/RNA Kit B is commonly used for pathogen nucleic acid extraction in targeted sequencing workflows such as tNGS, where stable nucleic acid recovery is required prior to library preparation. For sequencing strategies that benefit from reduction of host nucleic acid background, laboratories may consider MagPure Pathogen DNA/RNA Enrich Kit (R6672C).

Specifications

Extraction Principle

The MagPure Pathogen DNA/RNA Kit B uses magnetic bead adsorption chemistry to purify nucleic acids released from lysed biological samples. Under chaotropic binding conditions, nucleic acids bind to the surface of magnetic particles while proteins, cell debris and other contaminants remain in the solution.

After magnetic separation, the bound nucleic acids are washed to remove residual impurities and salts. The purified pathogen DNA and RNA are then eluted in nuclease-free buffer for downstream molecular analysis.

Engineering Features

The kit is designed to provide stable nucleic acid recovery across a range of biological samples with low microbial abundance. Optimized bead-binding chemistry supports efficient recovery of both DNA and RNA molecules while maintaining compatibility with automated magnetic extraction instruments.

The magnetic bead workflow also allows flexible integration with laboratory automation systems and supports consistent purification performance for sequencing preparation workflows.

Kit Contents

Storage and Stability

Particles MPN9 and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

For guidance on selecting the most appropriate DNA extraction system based on sample type, input volume and workflow requirements:

👉 Magen Kits Selection Guide