Introduction
MagPure Pathogen DNA/RNA Kit B is a low-background pathogen DNA/RNA extraction system for tNGS-oriented and pathogen panel workflows. The kit combines SDS-assisted bead-tube disruption with MPN9 magnetic particle purification, supporting recovery of pathogen nucleic acids from plasma, serum, body fluid, homogenate suspension, culture solution, cell suspension and concentrated pathogen preparations.
Its key value lies in low-background reagent control and stronger direct pathogen disruption, helping reduce reagent-derived nucleic acid interference while improving nucleic acid release from mixed pathogen materials. R6672B is positioned as a direct extraction workflow for targeted sequencing preparation, rather than a host-background reduction or pathogen enrichment workflow. For mNGS workflows requiring human DNA background reduction, Magen provides MagPure Pathogen DNA/RNA Enrich Kit (R6672C).
Details
Workflow
Workflow Overview
The MagPure Pathogen DNA/RNA Kit B uses a low-background reagent system combined with magnetic bead–based purification for pathogen DNA/RNA extraction. After low-speed clarification when required, the sample is processed through SDS-assisted bead-tube disruption to support broader release of nucleic acids from mixed pathogen materials. The clarified lysate is then purified using MPN9 magnetic particles, followed by washing, drying and elution. This workflow is positioned for tNGS and pathogen panel–oriented applications where direct pathogen disruption and low reagent-derived nucleic acid background are important.
Sample Handling Logic
The workflow is suitable for pathogen-containing samples such as plasma, serum, body fluid, homogenate suspension, culture solution, cell suspension, soaking solution and concentrated pathogen solution. For cell-rich samples, low-speed clarification helps reduce excessive host-cell carryover while retaining liquid-phase pathogen material for direct disruption. For sputum or lavage samples, DTT liquefaction may be required before extraction. The workflow should be understood as a direct pathogen disruption route rather than a host-background reduction or pathogen enrichment workflow.
Time and Workflow Characteristics
Under typical manual operation by an experienced operator, the workflow is usually completed within about 50–65 minutes. Processing time depends mainly on sample conditioning, bead-tube disruption method and clarification requirements. Longer handling may be required when vortex-based disruption, DTT liquefaction or additional sample clarification is used. For detailed step-by-step conditions, workflow guidance and estimated processing times, please refer to the Workflow Note in the Download section.
Specifications
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Features
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Specifications
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Main Functions
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Extract Pathogen RNA/DNA from 0.5ml plasma, serum, body fluid, homogenate suspension, culture solution, cell suspension, soaking solution or concentrate pathogen solution for tNGS application.
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Applications
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RT-PCR，Real Time PCR, biochip analysis, NGS
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Products
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Pathogen DNA / RNA
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Purification method
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Polydisperse magnetic beads
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Purification technology
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Magnetic beads technology
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Process method
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Manual or automatic
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Sample type
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low/cell-free samples such as plasma, serum, body fluid, homogenate suspension, culture solution, cell suspension, soaking solution or concentrate pathogen solution
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Sample amount
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0.5 ml
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Adaptive instrument
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Nucleic acid extractor, pipetting workstation
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Kit Contents
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Contents
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R667200B
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R6672-02B
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Purification Times
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24 Preps
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96 Preps
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2ml Bead Tubes
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24
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96
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Proteinase K
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12 mg
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50 mg
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Protease Dissolve Buffer
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1.8 ml
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3 ml
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Buffer SDS (20%)
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1.8 ml
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6 ml
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Particles MPN9
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0.6 ml
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2.5 ml
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Buffer MLB
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15 ml
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60 ml
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Buffer MW1*
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13 ml
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44 ml
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Buffer MW2*
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6 ml
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50 ml
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Buffer AVE
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5 ml
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30 ml
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Storage and Stability
Particles MPN9 and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
Purchase Guide
For guidance on selecting the most appropriate viral or pathogen nucleic acid extraction system based on sample type, target nucleic acid, automation needs and downstream application:
👉 Viral & Pathogen Nucleic Acid Extraction Kit Selection Guide
For a broader technical overview of Magen viral / pathogen workflow routes, background-control logic and PCR, tNGS or mNGS application orientation:
👉 Magen Viral / Pathogen Workflows Explained
For detailed workflow notes covering representative pathogen extraction and enrichment routes:
👉 IVD6672 Pathogen DNA/RNA Extraction Workflow Note
👉 R6672B Low-Background Pathogen DNA/RNA Extraction Workflow Note
👉 R6672C Host-Background Reduction and Pathogen Enrichment Workflow Note
👉 IVD4179 Column-Based Pathogen Enrichment Workflow Note
