The HiPure Paxgene Blood RNA Kit is designed for RNA purification from blood samples stabilized using PAXgene blood RNA collection tubes. The workflow is compatible with stabilized whole blood samples and supports RNA purification for transcriptomic analysis.

The protocol enables efficient RNA recovery from stabilized blood samples and is commonly used in gene expression profiling studies where RNA stabilization during sample collection is required.

For automated blood RNA purification workflows laboratories may refer to the MagPure Blood RNA Kit (R6611).

Specifications

Principle

The Kit is for the purification of total RNA from 2.5 ml human whole blood collected in a PAXgene Blood RNA Tube. Purification begins with a centrifugation step to pellet nucleic acids in the PAXgene Blood RNA Tube. The pellet is washed and resuspended, and incubated in optimized buffers together with proteinase K. DNA wash The lysate is passed through a DNA Mini column. Ethanolis added to adjust binding conditions, and the lysate is applied to a column.RNA is selectively bound to the silica membrane as contaminants pass through. Remaining contaminants are removed in several efficient wash steps. Between the first and second wash steps, the membrane is treated with DNase I to remove trace amounts of bound DNA. After the wash steps, RNA is eluted in elution buffer and heat-denatured.

Advantages

• High quality - high purity total RNA can be directly used in various sensitive downstream applications
• Fast - isolation of several samples can be completed in 40 minutes by using column purification method
• Safety - no phenol chloroform extraction required
• Sensitive - direct lysis of blood, plasma and other samples without separation of leukocytes

Kit Contents

Storage and Stability

Proteinase K should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

Experiment Data

Choosing the appropriate RNA extraction system depends on sample type, RNA species and workflow requirements.

For detailed selection guidance across blood and liquid RNA workflows, refer to the Blood RNA Kits Selection Guide.