Introduction
The MagPure DNA Micro Kit is designed for purification of genomic DNA from low-volume or trace biological samples using magnetic bead technology. The high-binding magnetic particles enable efficient recovery of DNA from small input volumes such as limited blood samples, forensic traces, or small tissue materials.
The system supports automated extraction workflows and is suitable for laboratories processing challenging samples where DNA quantity is limited.
For specialized forensic workflows laboratories may refer to the HiPure DNA Micro Kit (D3125) or the dedicated MagPure Forensic DNA Kit (D6359D), while routine extraction from standard biological samples can be performed using the MagPure Universal DNA Kit (IVD3102).
Details
Specifications
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Features
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Specifications
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Main Functions
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Isolation total DNA from 1-100ul blood, FFPE, tissue and other samples
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Applications
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Second generation sequencing, PCR, real timePCR, etc.
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Purification method
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Monodisperse magnetic beads
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Purification technology
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Magnetic beads technology
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Process method
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Manual or automatic
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Sample type
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Anticoagulant blood, concentrated blood, buffy coat, lymphocytes and cultured cells
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Sample amount
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Body fluid : 10 - 200μl, Tissue : ≤10mg
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Yield
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10ng - 15μg
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Elution volume
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80 -100μl
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Time per run
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Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Technical Validation
A performance validation was performed to evaluate the MagPure DNA Micro Kit and its IVD3101-TL-06 prefilled automated format using limited FFPE curl samples as a low-input DNA extraction model. Manual extraction and automated extraction on two instruments were compared over three consecutive days.
Purified DNA was assessed by NanoDrop, Quantus fluorometric quantification and a 116-panel downstream sequencing assay. The extracted DNA showed A260/280 values mainly within 1.7–2.0 and A260/230 values generally within 1.5–2.5, indicating acceptable purity for low-input DNA purification under the tested conditions.
Quantus quantification showed DNA concentrations within the tested library preparation requirement. Although NanoDrop readings varied between manual and automated formats due to platform, sampling and elution-volume differences, the overall DNA recovery was suitable for downstream library construction.
The 116-panel sequencing data showed good consistency between manual and automated extraction groups. Key sequencing quality metrics, including clean data output, Q20/Q30 values, mapping rate and reads-on-target performance, remained comparable across the tested extraction formats.
These results support the use of the MagPure DNA Micro Kit as a magnetic bead-based DNA extraction system for low-input samples, with compatibility for automated prefilled workflows and downstream targeted sequencing analysis.
Kit Contents
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Contents
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IVD3101
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Purification Times
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200
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MagBind Particles
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4.5 ml
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Proteinase K
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90 mg
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Protease Dissolve Buffer
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10 ml
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Buffer ATL
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60 ml
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Buffer AL
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60 ml
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Buffer BD*
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20 ml
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Buffer BXW1*
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110 ml
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Elution Buffer
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30 ml
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Storage and Stability
Proteinase K, MagBind Particles should be stored at 2–8°C upon arrival. However, short-termstorage (up to 18 weeks) at room temperature (15–25°C) does not affect their performance. The remainingkit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
