Introduction
HiPure Plasmid Mini Kit represents thestandard column reference model within the Magen plasmid DNA extractionportfolio. Developed for routine 1–5 mL bacterial culture workflows, it usesalkaline lysis followed by silica membrane purification to support everydayplasmid preparation for cloning, PCR, restriction digestion and standardsequencing work. The system is positioned as the core manual mini-prep formatwithin the standard plasmid branch.
Within this system, HiPure Plasmid Mini KitII (P1002) extends the workflow toward larger mini-prep culture input, while HiPure Plasmid Plus 96 Kit (P1006) converts the same standard branch into a96-well high-throughput format. Laboratories requiring low-endotoxin plasmidpreparation should move to the EF branch, with P1154, P1231 and P1156B coveringEF workflows at increasing preparation scale.
Details
Specifications
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Features
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Specifications
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Main Functions
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Isolation up to 35μg plasmid DNA from 1-5ml bacterial culture
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Applications
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Enzyme digestion, sequencing, PCR, cloning, etc.
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Purification method
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Mini spin column
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Purification technology
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Silica technology
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Process method
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Manual (centrifugation or vacuum)
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Sample type
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Conventional plasmid, plasmid less than 30KB
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Sample amount
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High copy plasmid: 1-5ml culture medium
Low copy number plasmid : 5-10ml culture medium
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Yield
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5-35µg
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Elution volume
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≥30μl
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Time per run
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Complete 1-24 samples in 30 minutes
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Liquid carrying volume per column
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800µl
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Binding yield of column
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35µg
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Extraction Principle
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Engineering Characteristics
Standard mini-prep membrane workflow
The kit is designed around a silica membrane mini-column format for routine 1–5mL bacterial culture processing, with protocol guidance also supportinglow-copy plasmids or cosmids when input conditions are adjusted appropriately.
Defined binding capacity for routinemini-prep use
Internal testing showed that plasmid recovery increased with culture input andbegan to level off at around 4–5 mL, supporting a practical column bindingcapacity of approximately 35 μg for this mini-prep format.
Optional background-control wash
An additional PW1 wash step is recommended when lower background contaminationor more controlled downstream performance is needed.
Technical Validation
Stable yield and purity across replicate preparations
Internal validation using 48 replicate 2 mL plasmid preparations showed stable yield variation within about 10%, with A260/280 around 1.81–1.92 and A260/230 around 2.0–2.4.
Downstream compatibility
Purified plasmid DNA supported restriction digestion and sequencing analysis in internal testing, confirming compatibility with routine molecular biology workflows.
Comparable small-scale performance
In internal comparison across common routine plasmid vectors, P1001 showed purity and yield performance in the same practical range as several widely used mini-prep kits.
Kit Contents
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Contents
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P100102
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P100103
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Purification Times
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100 Preps
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250 Preps
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RNase A
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5 mg
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10 mg
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Buffer P1
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30 ml
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80 ml
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Buffer P2
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30 ml
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80 ml
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Buffer P3
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40 ml
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100 ml
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Buffer PW1
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60 ml
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140 ml
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Buffer PW2*
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20 ml
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50 ml
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Elution Buffer
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15 ml
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30 ml
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HiPure DNA Mini Columns II
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100
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250
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2 ml Collection Tubes
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100
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250
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Storage and Stability
The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2-8°C.
Experiment Data
