Introduction
HiPure Plasmid EF Mini Kit is the mini-scale support configuration within the Magen low-endotoxin plasmid portfolio. It is designed for laboratories that require EF-grade plasmid DNA in a smaller column format, especially when preparation scale remains below routine midi or maxi workflows. In practical use, it is well suited to medium-to low-copy plasmids and moderate-input EF preparation where low-endotoxin control, accurate plasmid quantification and reduced RNA interference are all important.
Within the EF branch, P1154 serves as the compact support option below P1231 and P1156B. Laboratories that only need standard routine plasmid DNA should remain in the P1001 branch. When higher EF output is needed, HiPure Plasmid EF Midi Kit(P1231) provides the midi-scale step and HiPure Plasmid EF MaxiKit B (P1156B) remains the low-endotoxin column reference model for larger preparation volumes.
Details
Specifications
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Features
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Specifications
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Main Functions
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Isolation up to 80µg endotoxin-free plasmid DNA from 5-15ml bacterial culture. Better suited to low-copy plasmids and small-scale preparation with better RNA control.
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Applications
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Cell transfection, animal injection, etc.
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Purification method
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Mini spin column
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Purification technology
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Silica technology
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Process method
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Manual (centrifugation or vacuum)
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Sample type
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Low copy plasmid vector
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Sample amount
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5-15ml LB
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Yield
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10-70μg
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Elution volume
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≥75μl
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Time per run
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≤40 minutes
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Liquid carrying volume per column
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800μl
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Binding yield of column
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70μg
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Extraction Principle
After alkaline lysis and neutralization,the cleared lysate is processed under high-salt binding conditions before being transferred to the compact column. This workflow is designed for stronger plasmid selectivity during purification, helping reduce RNA interference and support more accurate OD-based plasmid quantification in the final preparation. Endotoxin-control chemistry is applied during binding and washing to reduce endotoxin carryover for downstream transfection-oriented use.
Engineering Characteristics
High-salt selective purification
The workflow uses high-salt–mediated plasmid purification in a compact EF miniformat, which improves plasmid selectivity and helps reduce RNA interference in the final preparation.
Accurate plasmid quantification
Because RNA carryover is minimized, OD-based plasmid concentration is more consistent with electrophoretic band intensity, which is useful when accurate DNA quantification matters before transfection or other downstream analysis.
Defined loading boundary
Internal testing showed that the column has a practical upper binding limit of about 75–80 μg. This makes culture input selection especially important for higher-copy plasmids and is one of the main reasons the product is better positioned as an EF mini solution rather than a general high-yield page.
Technical Validation
Low-copy preparation behavior
Internal validation showed good yield linearity when low-copy plasmids were processed across 4, 6, 10 and 20 mL culture input, supporting the product’s use in medium- to low-copy EF mini workflows.
High-copy boundary
For high-copy plasmids, yield increased predictably at 1, 2 and 4 mL input, but once total DNA approached the column binding limit, further input increase no longer translated into proportional recovery. This is a useful selection boundary for page positioning.
Reduced RNA interference
In internal comparison with another low-endotoxin plasmid workflow, P1154 showed more realistic plasmid concentration in medium- to low-copy preparations, consistent with lower RNA interference rather than inflated OD-based readings.
Low-yield A260/230 boundary
At lower total plasmid output, A260/230 may appear lower because of residual salt or guanidine background, but this does not necessarily prevent downstream transfection-oriented use and should be interpreted together with electrophoretic band quality and actual workflow performance.
Kit Contents
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Contents
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P115402
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P115403
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Purification Times
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50 Preps
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250 Preps
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RNase A
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5 mg
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20 mg
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Buffer P1
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30 ml
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140 ml
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Buffer P2
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30 ml
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140 ml
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Buffer LEN3
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15 ml
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70 ml
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Buffer LN4
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50 ml
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250 ml
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Buffer LN5
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30 ml
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140 ml
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Buffer PW1
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30 ml
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140 ml
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Buffer PW2
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12 ml
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50 ml
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Elution Buffer
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15 ml
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30 ml
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HiPure DNA Mini Columns III
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50
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250
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2 ml Collection Tubes
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50
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250
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Storage and Stability
The kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2–8°C.
