The HiPure Total RNA Kit provides a single-column silica membrane workflow for purification of large RNA molecules (>200 nt) from cultured cells and soft tissues. The method relies on chaotropic lysis followed by selective RNA adsorption on a silica membrane and sequential washing steps to remove proteins, salts and other contaminants.

The streamlined workflow is widely used for routine RNA preparation in molecular biology laboratories and supports downstream applications such as RT-PCR, qPCR and gene expression analysis. The protocol is suitable for standard laboratory workflows requiring consistent RNA purification from cultured cells or soft animal tissues.

Within the Magen RNA extraction systems, laboratories requiring genomic DNA removal during RNA purification may refer to the HiPure Total RNA Plus Kit (R4111), while automated RNA purification workflows can be implemented using the MagPure Universal RNA Kit (IVD3020).

Specifications

Principle

HiPure RNA technology simplifies total RNA isolation. Samples are first lysed and then homogenized. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the HiPure silica membrane and RNA binds to the silica membrane, and all contaminants are efficiently washed away. For certain RNA applications that are sensitive to very small amounts of DNA, the residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment. Pure, concentrated RNA is eluted in water.

Advantages

• Efficient removal of DNA - unique genomic DNA removal column without DNase treatment
• High quality - high purity total RNA can be directly used in various sensitive downstream applications
• Fast - several samples can be extracted in 20 minutes by column method
• Safe - no phenol chloroform extraction required
• Sensitive - RNA can be recovered at the level of PG

Kit Contents

Storage and Stability

The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Choosing the appropriate RNA extraction kits depends on sample type, RNA species and workflow requirements.

For detailed guidance across tissue and cell RNA extraction workflows, refer to the Tissue/Cell RNA Kits Selection Guide.